- Patent Number:
10145,833
- Appl. No:
10/468691
- Application Filed:
February 21, 2002
- نبذة مختصرة :
A method of analysis of a hydrocarbon fuel for the presence of a micro-organism comprises contacting a fuel sample with an aqueous diluent and with an antibody reactive with the micro-organism, or reactive with a metabolite or breakdown product produced by the micro-organism, to detect the presence or absence of the micro-organism.
- Inventors:
Kelley, Joan (Guilford, GB)
- Assignees:
CONIDIA BIOSCIENCE LIMITED (GB)
- Claim:
1. A method of analysis of a sample of a liquid hydrocarbon fuel for the presence of Hormoconis resinae , the method comprising the steps of: 1) mixing the liquid fuel sample with an aqueous diluent containing an emulsifier; 2) contacting the mixture or one phase thereof with an antiserum comprising antibodies raised against a homogenized preparation of Hormoconis resinae , wherein the Hormoconis resinae in the homogenized preparation was grown in contact with the liquid hydrocarbon fuel; and 3) detecting the presence or absence of bound antibodies in the sample, wherein the method provides a result within two hours from step 1, and wherein the detection of bound antibodies indicates the presence of Hormoconis resinae which is actively growing in liquid hydrocarbon fuel.
- Claim:
2. The method of claim 1 , wherein the method comprises vigorously mixing the sample of fuel and the aqueous diluent before contacting the mixture with the antibodies.
- Claim:
3. The method of claim 1 , wherein the emulsifier is a non-ionic detergent.
- Claim:
4. The method of claim 3 , wherein the emulsifier is polyoxyethylene (20) sorbitan monolaurate.
- Claim:
5. The method of claim 4 , wherein the emulsifier is used at a concentration of from 1 to 10% by volume.
- Claim:
6. The method of claim 1 , wherein the hydrocarbon fuel is light-middle distillate fuel having a boiling point below 390° C.
- Claim:
7. The method of claim 1 , wherein the fuel sample is not filtered prior to the step of contacting the mixture or one phase thereof with the antibodies.
- Claim:
8. The method of claim 1 , wherein the antibodies are not incubated with a filter used to filter the sample of the liquid hydrocarbon fuel.
- Claim:
9. A kit for analyzing a sample of a liquid hydrocarbon fuel for the presence of Hormoconis resinae , the kit comprising: 1) a homogenized preparation of Hormoconis resinae , wherein the Hormoconis resinae in the homogenized preparation was grown in contact with liquid hydrocarbon fuel; 2) an antiserum comprising antibodies raised against a homogenized preparation of Hormoconis resinae , wherein the Hormoconis resinae in the homogenized preparation was grown in contact with the liquid hydrocarbon fuel; and 3) an aqueous diluent containing an emulsifier.
- Claim:
10. The kit of claim 9 , further including a substrate to which the antibodies are immobilized.
- Claim:
11. The kit of claim 10 , wherein the substrate is a microtiter plate.
- Claim:
12. The kit of claim 9 , further including a lateral flow device to which the antibodies are immobilized.
- Patent References Cited:
5658747 August 1997 Feldsine et al.
5665585 September 1997 Torkkeli et al.
6139775 October 2000 Thames
WO 00/53699 September 2000
- Other References:
The Conidia Bioscience Fuelstat(TM) resinae (Aviation) Diagnostic Kit. (C) ConidiaBioscience 2000-2003. cited by examiner
Taxonomic Details, species: Amorphotheca resinae. In, New Zealand Fungi. (C) Landcare Research 2001-2004. cited by examiner
Lopes, P.T.C. 1996. Use of Immunofluorescence to detect the fungus Hormoconis resinae in aviation kerosine. International Biodeterioration & Biodegradation 37: 37-40. cited by examiner
Greenspan et al. (Nature Biotechnology 17: 936-937, 1999). cited by examiner
Lopes et al. (International Biodeterioation and Biodegradation vol. 37, pp. 37-40, 1996). cited by examiner
Sheridan et al. (Tuatara: vol. 19, Issue 3, 1972, pp. 130-165). cited by examiner
Kwak et al. (Journal of Microbiology and Biotechnology vol. 9, No. 6, pp. 764-772). cited by examiner
Brigmon, RL, Franck, MM, Bray, JS, Scott, DF, Lanclos, KD, and Fliermans, CB, “Direct immunofluorescence and enzyme-linked immunosorbent assays for evaluating organic contaminant degrading bacteria,” Journal of Microbiological Methods, 32: 1-10, 1998. cited by applicant
Gaylarde, CC, Bento, FM, and Kelley, J, “Microbial Contamination of Stored Hydrocarbon Fuels and its Control,” Revista de Microbiologia, 30: 1-10, 1999. cited by applicant
Lopes, PTC and Gaylarde, C, “Use of Immunofluorescence to Detect the Fungus Hormoconis resinae in Aviation Kerosine,” International Biodeterioration & Biodegradation, 37:37-40, 1996. cited by applicant
- Primary Examiner:
Zeman, Robert A
- Attorney, Agent or Firm:
McCarter & English, LLP
- الرقم المعرف:
edspgr.10145833
Processing Request
No Comments.