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Ice nucleation active microorganism

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  • Publication Date:
    October 26, 1999
  • معلومة اضافية
    • Patent Number:
      5,972,686
    • Appl. No:
      09/077,218
    • Application Filed:
      May 19, 1998
    • نبذة مختصرة :
      The present invention relates to a novel ice nucleation active Xanthomonas strain and a bacterial ice nucleator comprising the ice nucleation active microorganism which can be applied for food processing and artificial snow making. The present inventors have screened ice nucleation active microorganisms from leaves of crops and plants, and investigated their ice-nucleation activities. As a result, the inventors discovered that a novel microorganism belonging to Xanthomonas campestris has a superior ice-nucleation activity than those of the conventional ice nucleation active microorganisms. Accordingly, the ice nucleation active microorganism of the invention can be used as a potent bacterial ice nucleator for food processing and artificial snow making.
    • Inventors:
      Kang, Choong-Kyung (Taejon, KRX); Na, Gwang-Hwee (Taejon, KRX); Yoon, Hyun-Geun (Taejon, KRX); Hong, Seung-Suh (Taejon, KRX); Lee, Hyun-Soo (Taejon, KRX)
    • Assignees:
      Samyang Genex Co., Ltd. (Seoul, KRX)
    • Claim:
      What is claimed is
    • Claim:
      1. Xanthomonas campestris.
    • Claim:
      2. A bacterial ice nucleator which comprises Xanthomonas campestris KCTC 0251BP, and an appropriate carrier.
    • Claim:
      3. The bacterial ice nucleator of claim 2 wherein the carrier is suitable for food processing.
    • Claim:
      4. The bacterial ice nucleator of claim 2 wherein the carrier is suitable for artificial snow making.
    • Claim:
      5. A method for forming ice nuclei, comprising the steps of
    • Claim:
      contacting a subject containing liquid water with Xanthomonas campestris KCTC 0251BP; and
    • Claim:
      bacterially forming ice nuclei from the liquid water in the subject at super-cooling temperatures.
    • Claim:
      6. The method according to claim 5, wherein the super-cooling temperature is in the range of -2.degree. to -4.degree. C.
    • Current U.S. Class:
      4352/521; 424/934; 426/61; 435/168; 435/262; 435/910
    • Current International Class:
      C12N 120
    • Patent References Cited:
      4464473 August 1984 Orser et al.
      5194269 March 1993 Lee
      5489521 February 1996 So et al.
    • Other References:
      Li. J. et al., Bacterial Ice Nucleation and Its Potential Application in the Food Industry, Trends in Food Science & Technology, 6:259-265(1995).
      Watanabe M. et al., Screening, Isolation, and Identification of Food-originated Compounds Enhancing the Ice-nucleation Activity of Xanthomonas Campestris, Biosci. Biotech. Biochem., 58(1):64-66(1994).
      Kawahara H. et al., Isolation and Characterization of a Novel Ice-nucleation Bacterium, Pseudomonas sp. KUIN-4 Which Has Stable Activity in Acidic Solution, Biosci. Biotech. Biochem., 59(8):1528-1532(1995).
      Watanabe M. et al., Enhancing Effect of 4-Hydroxy-3-nitrophenylacetic Acid on Transcription of the Ice Nucleation-active Gene of Xanthomonas campestris, Biosci. Biotech. Biochem., 58(12):2269-2270(1994).
    • Primary Examiner:
      Lilling, Herbert J.
    • Attorney, Agent or Firm:
      Knobbe, Matens, Olson & Bear, LLP
    • الرقم المعرف:
      edspgr.05972686