- Patent Number:
4,308,375
- Appl. No:
06/194,516
- Application Filed:
October 06, 1980
- نبذة مختصرة :
A method for purifying water-insoluble polyene antibiotics, such as amphotericin and nystatin, is provided which method includes the step of passing a mixture of the polyene antibiotic dispersed in methanol through an ion exchange column whereby gram positive and gram negative bacteria are removed from the antibiotic.
- Inventors:
Tang, William W. (Mercerville, NJ)
- Assignees:
E. R. Squibb & Sons, Inc. (Princeton, NJ)
- Claim:
What is claimed is
- Claim:
1. A method for purifying water-insoluble polyene antibiotics, which comprises forming a slurry of the antibiotic in an appropriate solvent, passing the antibiotic-solvent mixture through an ion exchange resin to filter gram positive bacteria and gram negative bacteria from the antibiotic and recovering purified antibiotic from the ion exchange resin.
- Claim:
2. The method as defined in claim 1 wherein said antibiotic is amphotericin B.
- Claim:
3. The method as defined in claim 1 wherein said antibiotic is nystatin or pleuromutilin.
- Claim:
4. The method as defined in claim 1 wherein the solvent employed is methanol.
- Claim:
5. The method as defined in claim 1 wherein the antibiotic-solvent mixture is amphotericin B rich methanol.
- Claim:
6. The method as defined in claim 5 wherein the amphotericin B methanol mix has a pH of about 4 before it is passed through the ion exchange resin.
- Claim:
7. The method as defined in claim 2 wherein the ion exchange resin is type I quaternary ammonium anion ion exchange resin.
- Claim:
8. The method as defined in claim 3 wherein the ion exchange resin is type I quaternary ammonium anion ion exchange resin.
- Claim:
9. The method as defined in claim 1 wherein the amphotericin B rich methanol is passed through the ion exchange resin at a flow rate of from about 7 to about 10 gpm/ft.sup.3 of resin bed volume.
- Claim:
10. The method as defined in claim 4 wherein said antibiotic-solvent mixture contains from about 1 to about 5% by weight antibiotic.
- Claim:
11. The method as defined in claim 10 wherein the antibiotic-solvent mixture is at a temperature of within the range of from about 15.degree. to about 25.degree. C.
- Claim:
12. The method as defined in claim 1 wherein the antibiotic-solvent mixture is at a pH within the range of from about 3.5 to about 4.5.
- Claim:
13. The method as defined in claim 1 wherein the antibiotic-solvent mixture is passed through the ion exchange resin at a flow rate of within the range of from about 3 to about 10 gal/min/ft.sup.3 (resin bed volume).
- Current U.S. Class:
536/17R; 424/180
- Current International Class:
C07H 1708
- Patent References Cited:
3945993 March 1976 Schaffner et al.
4177265 December 1979 Michel et al.
- Other References:
Slejko, Chem. Absts., 90:76094n, p. 251 (1979), "Removal of Biological Matter from Aqueous Streams Via Ion Exchange Filtration".
Schubert, Chem. Absts., 85:83086m, p. 333 (1976), "On Bacterial Aftergrowth in Drinking and Industrial Water II. Apparative and Processing Effects on the Growth of Bacteria and the Possibility of Disinfection on Ion Exchange Resin Systems".
Kuehne, Chem. Absts., 74:130263b, p. 318 (1971), "Ion Exchange or Activated Charcoal Filter Contaminated with Microbial Impurities".
Rohm and Haas Co., Chem. Absts., 91:198601s, p. 354 (1979), "Bacteria Removal from Liquid Media by Ion Exchange Column".
Semmens, Chem. Absts., 90:11888e, p. 342 (1979), "Water Purification Process".
Ogoshi, Chem. Absts., 90:192358a, p. 350 (1979), "Ultrapure Water".
- Primary Examiner:
Brown, Johnnie R.
- Attorney, Agent or Firm:
Levinson, Lawrence S.
Rodney, Burton
- الرقم المعرف:
edspgr.04308375
Processing Request
No Comments.