- Patent Number:
4,285,976
- Appl. No:
06/096,789
- Application Filed:
November 23, 1979
- نبذة مختصرة :
Autolysis of yeast slurries is accelerated by the presence of thiamine and/or pyridoxine and by gradually raising the temperature of the yeast slurry to the incubation temperature over a period of time of from about 20 to about 180 minutes.
- Inventors:
Akin, Cavit (Naperville, IL); Murphy, Rose M. (Naperville, IL)
- Assignees:
Standard Oil Company (Indiana) (Chicago, IL)
- Claim:
We claim
- Claim:
1. In a process for autolyzing yeasts wherein a yeast slurry is incubated at a temperature of from about 40.degree. to about 60.degree. C. to solubilize the yeast cell materials, the improvement comprising incubating the yeast slurry for from about 2 to about 16 hours in admixture with an additive selected from the group consisting of thiamine, pyridoxine, and a combination of thiamine and pyridoxine at a concentration of at least 0.01 weight percent.
- Claim:
2. The process of claim 1 wherein the concentration of the additive is from about 0.01 to about 0.4 weight percent.
- Claim:
3. The process of claim 1 wherein the autolysis is carried out in the presence of thiamine.
- Claim:
4. The process of claim 3 wherein the concentration of thiamine is from about 0.05 to about 0.3 weight percent.
- Claim:
5. The process of claim 1 wherein the autolysis is carried out in the presence of pyridoxine.
- Claim:
6. The process of claim 5 wherein the concentration of pyridoxine is from about 0.05 to about 0.2 weight percent.
- Claim:
7. The process of claim 1 wherein the yeast slurry is gradually heated to the incubation temperature over the course of from about 20 to about 180 minutes.
- Claim:
8. The process of claim 1 wherein the yeast slurry is gradually heated to the incubation temperature over the course of from about 30 to about 60 minutes.
- Claim:
9. The process of claim 1 wherein the yeast slurry contains at least one plasmolyzing agent in addition to the additive.
- Claim:
10. The process of claim 9 wherein at least one of the plasmolyzing agents is salt.
- Claim:
11. A process for autolyzing an aqueous yeast slurry comprising incubating the slurry at a temperature of from about 50 to about 55.degree. C. for about 2 to about 16 hours in admixture with from about 1 to about 6 weight percent salt, from about 0.05 to about 4 weight percent ethanol, from about 0.05 to about 3 weight percent dextrose, and an additive selected from the group consisting of thiamine, pyridoxine, and a combination of thiamine and pyridoxine at a concentration of at least 0.01 weight percent.
- Claim:
12. The process of claim 11 wherein the slurry is gradually heated to the incubation temperature over a time span of from about 20 to about 180 minutes.
- Claim:
13. The process of claim 12 wherein the yeast is Saccharomyces cerevisiae.
- Current U.S. Class:
426/60; 426/589; 426/650; 435/256; 435/259; 435/267; 435/272
- Current International Class:
A23L 128; A23J 118; C12C 1134; C12N 106
- Patent References Cited:
1012147 December 1911 Nolf
2295036 September 1941 Gorcica et al.
2328025 August 1943 Mead, Jr. et al.
2922748 January 1960 Peppler et al.
3961080 June 1976 Sugimoto et al.
3975553 August 1976 Griffon
4066793 January 1978 Eguchi
4218481 August 1980 Chao et al.
- Other References:
Joslyn et al., Yeast Autolysis, Wellerstein Lab. Communications, vol. 18, No. 62, 1955 (pp. 191-201).
White, J., Yeast Technology, John Wiley & Sons, Inc., N. Y. 1954 (pp. 80-81).
- Primary Examiner:
Naff, David M.
- Attorney, Agent or Firm:
Croft, Gregory E.
McClain, William T.
Magidson, William H.
- الرقم المعرف:
edspgr.04285976
Processing Request
No Comments.