- Document Number:
20240352408
- Appl. No:
18/654976
- Application Filed:
May 03, 2024
- نبذة مختصرة :
The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.
- Claim:
1. A prepolymerization solution, comprising: poly (ethylene glycol) having a molecular weight of greater than 2,000 Daltons and comprising at least two acrylate groups per molecule of poly (ethylene glycol); a photo-initiator; a cell; and a biocompatible, light-absorbing additive material suitable to control light penetration.
- Claim:
2. The prepolymerization solution of claim 1, wherein the biocompatible, light-absorbing additive material is organic.
- Claim:
3. The prepolymerization solution of claim 1, wherein the biocompatible, light-absorbing additive material is tartrazine.
- Claim:
4. The prepolymerization solution of claim 1, wherein the biocompatible, light-absorbing additive material is curcumin.
- Claim:
5. The prepolymerization solution of claim 1, wherein the photo-initiator is lithium acylphosphinate.
- Claim:
6. The prepolymerization solution of claim 1, further comprising a water content of 10 wt % to about 99.5 wt %.
- Claim:
7. The prepolymerization solution of claim 1, further comprising a water content of 80 wt % to about 90 wt %.
- Claim:
8. The prepolymerization solution of claim 1, wherein the cell is transfected or transduced with a protein configured to absorb the light in the wavelength range.
- Claim:
9. The prepolymerization solution of claim 8, wherein the protein is a cyan fluorescent protein (CFP) or a green fluorescent protein (GFP).
- Claim:
10. The prepolymerization solution of claim 1, further comprising a cryoprotectant.
- Claim:
11. The prepolymerization solution of claim 10, wherein the cryoprotectant is a low molecular weight poly (ethylene glycol) (PEG), glycerol, ethylene glycol, sucrose, propylene glycol, trehalose, raffinose, guar gum, xanthan gum, or D-mannitol.
- Claim:
12. The prepolymerization solution of claim 1, further comprising a medium configured to provide nutrition and/or buoyancy for the cell.
- Claim:
13. The prepolymerization solution of claim 12, wherein the medium is a Dulbecco's modified eagle medium (DMEM), a serum, a protein, a growth factor, a thickening agent, or an anti-clumping component.
- Claim:
14. A prepolymerization solution, comprising: poly (ethylene glycol) having a molecular weight of greater than 2,000 Daltons and comprising at least two acrylate groups per molecule of poly (ethylene glycol); a photo-initiator; and a protein with a peak light absorption overlapping that of the photo-initiator.
- Claim:
15. The prepolymerization solution of claim 14, wherein the prepolymerization solution excludes a light-absorbing additive material.
- Claim:
16. The prepolymerization solution of claim 14, wherein the prepolymerization solution further comprises a biocompatible, light-absorbing additive material, wherein the biocompatible, light-absorbing additive material has an additional capability to cause printing, by controlling light penetration, a structure with horizontal and vertical channels.
- Claim:
17. The prepolymerization solution of claim 14, further comprising a cell, wherein the cell is transfected or transduced with the protein.
- Claim:
18. The prepolymerization solution of claim 14, wherein the protein is a cyan fluorescent protein (CFP) or a green fluorescent protein (GFP).
- Claim:
19. The prepolymerization solution of claim 14, further comprising a cryoprotectant.
- Claim:
20. The prepolymerization solution of claim 14, further comprising a medium configured to provide nutrition and/or buoyancy for the cell.
- Current International Class:
12; 29; 29; 29; 33; 33; 33; 12; 12; 12
- الرقم المعرف:
edspap.20240352408
Processing Request
No Comments.