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Glutamate-Cysteine Ligase Variant and Method of Producing Glutathione Using the Same

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  • Publication Date:
    July 11, 2024
  • معلومة اضافية
    • Document Number:
      20240229006
    • Appl. No:
      17/914306
    • Application Filed:
      March 24, 2021
    • نبذة مختصرة :
      Provided are a novel glutamate-cysteine ligase variant and a method of producing glutathione using the same.
    • Assignees:
      CJ CHEILJEDANG CORPORATION (Seoul, KR)
    • Claim:
      1. A glutamate-cysteine ligase variant in which an amino acid corresponding to the 86th position from the N-terminus of an amino acid sequence of SEQ ID NO: 1 is substituted with a different amino acid.
    • Claim:
      2. The glutamate-cysteine ligase variant of claim 1, wherein the amino acid corresponding to the 86th position is substituted with glycine, alanine, valine, leucine, isoleucine, methionine, phenylalanine, tryptophan, proline, serine, threonine, tyrosine, asparagine, glutamate, glutamine, aspartate, lysine, arginine, or histidine.
    • Claim:
      3. The glutamate-cysteine ligase variant of claim 1, wherein the variant has a sequence homology equal to or greater than 80% and less than 100% with the amino acid sequence of SEQ ID NO: 1.
    • Claim:
      4. The glutamate-cysteine ligase variant of claim 1, wherein the variant consists of one amino acid sequence selected from SEQ ID NOS: 3 to 21.
    • Claim:
      5. A polynucleotide encoding the glutamate-cysteine ligase variant according to claim 1.
    • Claim:
      6. A vector comprising the polynucleotide of claim 5.
    • Claim:
      7. A microorganism producing glutathione comprising at least one of: the glutamate-cysteine ligase variant according to claim 1; a polynucleotide encoding the variant; and a vector comprising the polynucleotide.
    • Claim:
      8. The microorganism of claim 7, wherein the microorganism is a microorganism belonging to the genus Saccharomyces.
    • Claim:
      9. The microorganism of claim 7, wherein the microorganism is Saccharomyces cerevisiae.
    • Claim:
      10. The microorganism of claim 7, wherein the microorganism is a Saccharomyces cerevisiae strain deposited with Accession No. KCCM12659P.
    • Claim:
      11. A method of producing glutathione, the method comprising culturing a microorganism comprising at least one of: the glutamate-cysteine ligase variant according to claim 1; a polynucleotide encoding the variant; and a vector comprising the polynucleotide, in a culture medium.
    • Claim:
      12. The method of claim 11, further comprising recovering glutathione from at least one selected from the cultured microorganism, a dried product of the microorganism, an extract of the microorganism, a culture of the microorganism, and a lysate of the microorganism.
    • Current International Class:
      12; 12; 12
    • الرقم المعرف:
      edspap.20240229006