- Document Number:
20240052360
- Appl. No:
17/642050
- Application Filed:
August 19, 2021
- نبذة مختصرة :
Provided are: a plant cell, in which expression or activity of a phytochelatin synthase (PCS) gene or a protein encoded by the PCS gene is reduced as compared with a parent cell; a plant having that has reduced heavy metal absorption, and includes the plant cell; a method of reducing heavy metals in a plant, the method including reducing expression or activity of a PCS gene or a protein encoded by the PCS gene, as compared with a parent cell; a CRISPR-Cas9 recombinant vector including a single guide RNA targeting a PCS gene; and a method of preparing a plant with reduced heavy metals, the method including transforming a plant cell with the recombinant vector.
- Assignees:
KT&G CORPORATION (Daejeon, KR)
- Claim:
1. A plant cell in which expression or activity of a phytochelatin synthase (PCS) gene or a protein encoded by the PCS gene is reduced, as compared with a parent cell.
- Claim:
2. The plant cell of claim 1, wherein the PCS gene is a PCS gene derived from Nicotiana sylvestris (NtPCSs), a PCS gene derived from Nicotiana tomentosiformis (NtPCSt), or a combination thereof (NtPCSst).
- Claim:
3. The plant cell of claim 1, wherein the plant cell is genetically engineered by at least one selected from the group consisting of an RNA interference (RNAi) system, a meganuclease system, a zinc finger nuclease system, a transcription activator-like effector nuclease (TALEN) system, a CRISPR/Cas9 system, X-ray irradiation, gamma-ray irradiation, ethyl methanesulfonate treatment, and dimethyl sulfate treatment.
- Claim:
4. The plant cell of claim 1, wherein the plant is Nicotiana tabacum.
- Claim:
5. A plant having reduced heavy metal absorption, comprising the plant cell of claim 1.
- Claim:
6. A method of reducing heavy metals in a plant, the method comprising reducing expression or activity of a PCS gene or a protein encoded by the PCS gene, as compared with a parent cell of the plant.
- Claim:
7. The method of claim 6, wherein the reducing of expression or activity of the PCS gene or the protein encoded by the PCS gene is performed by at least one selected from the group consisting of an RNA interference (RNAi) system, a meganuclease system, a zinc finger nuclease system, a transcription activator-like effector nuclease (TALEN) system, a CRISPR/Cas9 system, X-ray irradiation, gamma-ray irradiation, ethyl methanesulfonate treatment, and dimethyl sulfate treatment.
- Claim:
8. The method of claim 6, wherein the heavy metal is one or more selected from the group consisting of cadmium, arsenic, antimony, lead, mercury, chromium, tin, zinc, barium, bismuth, nickel, cobalt, manganese, iron, copper, and vanadium.
- Claim:
9. A CRISPR-Cas9 recombinant vector comprising a single guide RNA targeting a PCS gene.
- Claim:
10. The CRISPR-Cas9 recombinant vector of claim 9, wherein the PCS gene is a PCS gene derived from Nicotiana sylvestris (NtPCSs) or a PCS gene derived from Nicotiana tomentosiformis (NtPCSt).
- Claim:
11. The CRISPR-Cas9 recombinant vector of claim 10, wherein the sgRNA targeting the NtPCSs gene consists of a nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 2, the sgRNA targeting the NtPCSt gene consists of a nucleotide sequence of SEQ ID NO: 3 or SEQ ID NO: 4, and the sgRNA targeting the NtPCSs gene and the NtPCSt gene consists of a nucleotide sequence of SEQ ID NO: 5 or SEQ ID NO: 6.
- Claim:
12. A method of preparing a plant having reduced heavy metal absorption, the method comprising transforming a plant cell with the recombinant vector of claim 9.
- Current International Class:
12; 12; 12; 12
- الرقم المعرف:
edspap.20240052360
Processing Request
No Comments.