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C-KIT-POSITIVE BONE MARROW CELLS AND USES THEREOF

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اقرأ أكثر حفظ في قائمتي
  • Publication Date:
    August 17, 2023
  • معلومة اضافية
    • Document Number:
      20230256022
    • Appl. No:
      18/084206
    • Application Filed:
      December 19, 2022
    • نبذة مختصرة :
      Disclosed herein are compositions comprising myogenic bone marrow cells that are c-kit positive. Such compositions are useful for treating cardiac diseases or disorders. Also disclosed herein are methods of producing myogenic bone marrow cells are c-kit positive. Further disclosed are cardiopoietic genes having enhanced expression in c-kit positive myogenic bone marrow cells.
    • Assignees:
      AAL SCIENTIFICS, INC. (New York, NY, US)
    • Claim:
      1. A method of treating or preventing a heart disease or disorder in a subject in need thereof comprising administering isolated myogenic bone marrow cells to the subject, wherein the myogenic bone marrow cells are c-kit positive (c-kit-BMCs).
    • Claim:
      2. The method of claim 1, wherein the heart disease or disorder is heart failure, diabetic heart disease, rheumatic heart disease, hypertensive heart disease, ischemic heart disease, cerebrovascular heart disease, inflammatory heart disease and/or congenital heart disease.
    • Claim:
      3. The method of claim 1, wherein the c-kit-BMCs are a subpopulation of c-kit positive bone marrow cells isolated from bone marrow.
    • Claim:
      4. The method of claim 1, wherein the c-kit-BMCs are able to transdifferentiate into cardiomyocytes, endothelial cells, fibroblasts, coronary vessels and/or cells of mesodermal origin.
    • Claim:
      5. The method of claim 1, wherein the c-kit-BMCs have enhanced expression of cardiopoietic genes compared to non-myogenic c-kit positive bone marrow cells.
    • Claim:
      6. The method of claim 1, wherein the c-kit-BMCs have enhanced expression of RYR3, OSM, Jag1, Hey2 and Smyd3 compared to non-myogenic c-kit positive bone marrow cells.
    • Claim:
      7. A method of repairing and/or regenerating damaged tissue of a heart in a subject in need thereof comprising: (a) extracting c-kit positive bone marrow cells from bone marrow; (b) selecting myogenic c-kit positive bone marrow cells (c-kit-BMCs) from step (a); (c) culturing and expanding said c-kit-BMCs from step (b); and (d) administering a dose of said c-kit-BMCs from step (c) to an area of damaged tissue in the subject effective to repair and/or regenerate the damaged tissue of the heart.
    • Claim:
      8. A method of producing myogenic c-kit positive bone marrow cells (c-kit-BMCs), comprising: (a) isolating c-kit positive bone marrow cells from bone marrow; (b) selecting myogenic c-kit positive bone marrow cells (c-kit-BMCs) from step (a); and (c) culturing and expanding the c-kit-BMCs of step (b), thereby producing c-kit-BMCs.
    • Claim:
      9. The method of claim 7, wherein the selecting step comprises selecting c-kit-BMCs having enhanced expression of RYR3, OSM, Jag1, Hey2 and Smyd3.
    • Claim:
      10. The method of claim 9, wherein the selecting step comprises selecting c-kit-BMCs having enhanced expression of RYR3, OSM, Jag1, Hey2 and Smyd3.
    • Current International Class:
      61; 12; 12
    • الرقم المعرف:
      edspap.20230256022