- Document Number:
20230193514
- Appl. No:
18/150519
- Application Filed:
January 05, 2023
- نبذة مختصرة :
A probiotic-encapsulating gum Arabic (GA) composite fiber/capsule, including: lactic acid bacteria and an electrospun fiber or electrosprayed capsule, where the lactic acid bacteria are encapsulated in the electrospun fiber or electrosprayed capsule by electro-hydro dynamics (EHD) technology. The electrospun fiber or electrosprayed capsule is prepared by compounding a polymer matrix with GA, and the polymer matrix is polyvinyl alcohol (PVOH), polyvinylpyrrolidone (PVP), whey protein concentrate (WPC) or maltodextrin (MD). A preparation method and an application of the composite fiber/capsule are also provided.
- Claim:
1. A probiotic-encapsulating gum Arabic (GA) composite fiber/capsule, comprising: lactic acid bacteria; and an electrospun fiber or an electrosprayed capsule; wherein the lactic acid bacteria are encapsulated in the electrospun fiber or electrosprayed capsule; the electrospun fiber or electrosprayed capsule is prepared by compounding a biopolymer matrix with GA; and the biopolymer matrix is selected from the group consisting of polyvinyl alcohol, polyvinylpyrrolidone and whey protein; when the biopolymer matrix is polyvinyl alcohol or polyvinylpyrrolidone, the probiotic-encapsulating GA composite fiber/capsule is prepared through steps of: (a1) preparing a suspension of the lactic acid bacteria; dissolving GA powder in water to obtain a GA solution; dissolving the polyvinyl alcohol or polyvinylpyrrolidone in water to obtain a biopolymer solution; and mixing the biopolymer solution with the GA solution uniformly to obtain a first mixed solution; (a2) adding the suspension of the lactic acid bacteria to the first mixed solution followed by uniform stirring to obtain a second mixed solution; and (a3) adding an additive to the second mixed solution followed by electrospinning to obtain the probiotic-encapsulating GA composite fiber or electrospraying to obtain the probiotic-encapsulating GA composite capsule; when the biopolymer matrix is the whey protein, the probiotic-encapsulating GA composite fiber/capsule is prepared through steps of: (b1) preparing a suspension of the lactic acid bacteria; dissolving GA powder in water to obtain a GA solution; adding the whey protein to the GA solution followed by mixing and stirring to obtain a first mixed solution; (b2) adding the suspension of the lactic acid bacteria to the first mixed solution followed by uniform stirring to obtain a second mixed solution; and (b3) adding an additive to the second mixed solution followed by electrospinning to obtain the probiotic-encapsulating GA composite fiber or electrospraying to obtain the probiotic-encapsulating GA composite capsule; and in step (a3), the additive is tween 80, and is 5% of a weight of the polyvinyl alcohol or polyvinylpyrrolidone in the second mixed solution; and in step (b3), the additive is tween 80, and is 5% of a weight of the whey protein in the second mixed solution.
- Claim:
2. The probiotic-encapsulating GA composite fiber/capsule of claim 1, wherein the lactic acid bacteria are Lactobacillus plantarum KLDS 1.0328.
- Claim:
3. The probiotic-encapsulating GA composite fiber/capsule of claim 1, wherein a diameter of the electrospun fiber is 150 \~170 nm, and a diameter of the electrosprayed capsule is 0.8 \~1.2 µm.
- Claim:
4. The probiotic-encapsulating GA composite fiber/capsule of claim 1, wherein in the steps (a1) and (b1), the suspension of the lactic acid bacteria is prepared through the following steps: inoculating the lactic acid bacteria into a deMan, Rosa and Sharpe (MRS) liquid medium at an inoculum amount of 2%, followed by anaerobic culture at 35~39° C. for 20 \~24 h and centrifugation at 4° C. and 5000 × g for 10 min to collect cells; and washing the cells with a sterile phosphate-buffered saline (PBS) followed by resuspending with the PBS to a cell concentration of 109 \~1010 CFU/mL to produce the suspension of the lactic acid bacteria.
- Claim:
5. The probiotic-encapsulating GA composite fiber/capsule of claim 1, wherein in the step (a1), a weight/volume percent concentration of the GA solution is 20%; a weight/volume percent concentration of the biopolymer solution is 10%; and a weight ratio of the biopolymer solution to the GA solution is 8: 2; and in the step (b1), a weight/volume percent concentration of the GA solution is 4%, and a weight/volume percent concentration of the whey protein in the first mixed solution is 20%.
- Claim:
6. The probiotic-encapsulating GA composite fiber/capsule of claim 1, wherein in the step (a2), a concentration of the lactic acid bacteria in the second mixed solution is 109 \~ 1010 CFU/mL; and in the step (b2), a concentration of the lactic acid bacteria in the second mixed solution is 109 \~1010 CFU/mL.
- Claim:
7. The probiotic-encapsulating GA composite fiber/capsule of claim 1, wherein in the steps (a3) and (b3), the electrospinning or the electrospraying is carried out at a voltage of 16 \~21 kV, a flow rate of 0.3-1.0 mL/h, and a distance of 10 \~16 cm.
- Claim:
8. An application of the GA composite fiber/capsule of any one of claims 1-7 in preparation offood or pharmaceuticals or health products.
- Current International Class:
01; 61; 12; 12; 01
- الرقم المعرف:
edspap.20230193514
Processing Request
No Comments.