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METHOD FOR DETECTING MYCOBACTERIUM TUBERCULOSIS

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اقرأ أكثر حفظ في قائمتي
  • Publication Date:
    June 22, 2023
  • معلومة اضافية
    • Document Number:
      20230193346
    • Appl. No:
      17/923403
    • Application Filed:
      April 29, 2021
    • نبذة مختصرة :
      The present invention relates to a method for detecting Mycobacterium tuberculosis, which can replace the conventional culture method that takes a long time of four to eight weeks to detect active tuberculosis, and which is a method for detecting active Mycobacterium tuberculosis by using isotopes on a sample of a patient's sputum or bronchoalveolar lavage fluid.
    • Claim:
      1. A composition for detecting Mycobacterium tuberculosis in sputum or bronchoalveolar lavage fluid, comprising urea (13C-urea or 14C-urea) composed of a carbon isotope.
    • Claim:
      2. The composition of claim 1, wherein the Mycobacterium tuberculosis is active Mycobacterium tuberculosis.
    • Claim:
      3. The composition of claim 1, wherein the composition detects a culture product for 1 to 7 days as a target.
    • Claim:
      4. A method for detecting Mycobacterium tuberculosis, comprising the steps of: (a) treating a biological sample isolated from a patient suspected of tuberculosis with NaOH or N-acetyl-L-cysteine (NALC)-NaOH; (b) culturing the sample treated with NaOH or N-acetyl-L-cysteine (NALC)-NaOH in a liquid medium for culturing Mycobacterium tuberculosis; (c) treating a culture product cultured in the liquid medium with urea (13C-urea or 14C-urea) composed of a carbon isotope; and (d) measuring 13CO2 or 14CO2 of the culture product.
    • Claim:
      5. The method of claim 4, wherein the biological sample is sputum or bronchoalveolar lavage fluid.
    • Claim:
      6. The method of claim 4, wherein the pH of the culture product cultured in the liquid medium of step (c) is pH 5 to pH 8.
    • Claim:
      7. A method for providing information for diagnosing tuberculosis, comprising the steps of: (a) treating a biological sample isolated from a patient suspected of tuberculosis with NaOH or N-acetyl-L-cysteine (NALC)-NaOH; (b) culturing the sample treated with NaOH or N-acetyl-L-cysteine (NALC)-NaOH in a liquid medium for culturing Mycobacterium tuberculosis; (c) treating a culture product cultured in the liquid medium with urea (13C-urea or 14C-urea) composed of a carbon isotope; (d) measuring 13CO2 or 14CO2 of the culture product; and (e) determining the patient as a tuberculosis patient if the level of 13CO2 or 14CO2 is higher than a normal sample.
    • Claim:
      8. The method of claim 7, wherein the biological sample is sputum or bronchoalveolar lavage fluid.
    • Current International Class:
      12; 12
    • الرقم المعرف:
      edspap.20230193346