Methods for Degrading or Converting Cellulosic Material

20130309723

13/982857

March 26, 2012

Provided are methods for degrading or converting a cellulosic material, comprising: treating the cellulosic material with an enzyme composition in the presence of a polypeptide having catalase activity; and enzyme composition used for degrading or converting a cellulosic material comprising one or more (e.g., several) enzymes having cellulolytic and/or hemicellulolytic activity and a polypeptide having catalase activity.

NOVZYMES A/S (Bagsvaerd, DK)

1-25. (canceled)

26. A method for degrading or converting a cellulosic material, comprising: treating the cellulosic material with an enzyme composition in the presence of a polypeptide having catalase activity.

27. The method of claim 26, wherein the enzyme composition comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.

28. The method of claim 26, wherein the cellulosic material is selected from the group consisting of agricultural residue, herbaceous material, municipal solid waste, pulp and paper mill residue, waste paper, and wood; preferably, arundo, bagasse, bamboo, corn cob, corn fiber, corn stover, miscanthus, orange peel, rice straw, switchgrass, wheat straw, eucalyptus, fir, pine, poplar, spruce, willow, algal cellulose, bacterial cellulose, cotton linter, filter paper, microcrystalline cellulose, or phosphoric-acid treated cellulose.

29. The method of claim 26, wherein the cellulosic material is pretreated, especially by chemical pretreatment, physical pretreatment, or biochemical pretreatment.

30. The method of claim 26, wherein the polypeptide having catalase activity is selected from the group consisting of: (a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO: 2, the mature polypeptide of SEQ ID NO: 4, the mature polypeptide of SEQ ID NO: 6, or the mature polypeptide of SEQ ID NO: 8; (b) a polypeptide encoded by a polynucleotide that hybridizes under low, medium, medium-high, high, or very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, or SEQ ID NO: 7, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii); (c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, the mature polypeptide coding sequence of SEQ ID NO: 3, the mature polypeptide coding sequence of SEQ ID NO: 5, or the mature polypeptide coding sequence of SEQ ID NO: 7; or the cDNA sequence thereof; (d) a variant of the mature polypeptide of SEQ ID NO: 2, a variant of the mature polypeptide of SEQ ID NO: 4, a variant of the mature polypeptide of SEQ ID NO: 6, or a variant of the mature polypeptide of SEQ ID NO: 8, comprising a substitution, deletion, and/or insertion at one or more positions; and (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has catalase activity.

31. A method for producing a fermentation product, comprising: (a) saccharifying a cellulosic material with an enzyme composition in the presence of a polypeptide having catalase activity; (b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and (c) recovering the fermentation product from the fermentation.

32. The method of claim 31, wherein the enzyme composition comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.

33. The method of claim 31, wherein the cellulosic material is selected from the group consisting of agricultural residue, herbaceous material, municipal solid waste, pulp and paper mill residue, waste paper, and wood; preferably, arundo, bagasse, bamboo, corn cob, corn fiber, corn stover, miscanthus, orange peel, rice straw, switchgrass, wheat straw, eucalyptus, fir, pine, poplar, spruce, willow, algal cellulose, bacterial cellulose, cotton linter, filter paper, microcrystalline cellulose, or phosphoric-acid treated cellulose.

34. The method of claim 31, wherein the fermentation product is an alcohol, an alkane, a cycloalkane, an alkene, an amino acid, a gas, isoprene, a ketone, an organic acid, or polyketide.

35. The method of claim 31, wherein the presence of the polypeptide having catalase activity increases the hydrolysis of the cellulosic material compared to the absence of the polypeptide having catalase activity.

36. The method of claim 31, wherein the polypeptide having catalase activity is selected from the group consisting of: (a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO: 2, the mature polypeptide of SEQ ID NO: 4, the mature polypeptide of SEQ ID NO: 6, or the mature polypeptide of SEQ ID NO: 8; (b) a polypeptide encoded by a polynucleotide that hybridizes under low, medium, medium-high, high, or very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, or SEQ ID NO: 7, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii); (c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1, the mature polypeptide coding sequence of SEQ ID NO: 3, the mature polypeptide coding sequence of SEQ ID NO: 5, or the mature polypeptide coding sequence of SEQ ID NO: 7; or the cDNA sequence thereof; (d) a variant of the mature polypeptide of SEQ ID NO: 2, a variant of the mature polypeptide of SEQ ID NO: 4, a variant of the mature polypeptide of SEQ ID NO: 6, or a variant of the mature polypeptide of SEQ ID NO: 8, comprising a substitution, deletion, and/or insertion at one or more positions; and (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has catalase activity.

37. An enzyme composition for degrading or converting a cellulosic material comprising one or more enzymes having cellulolytic and/or hemicellulolytic activity and a polypeptide having catalase activity.

38. The enzyme composition of claim 37, further comprising one or more enzymes selected from the group consisting of a GH61 polypeptide having cellulolytic enhancing activity, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.

435/72

12; 12; 12; 12; 12; 12; 12

edspap.20130309723