- Document Number:
20120184008
- Appl. No:
13/386894
- Application Filed:
August 05, 2010
- نبذة مختصرة :
The present invention relates to the production of a C-15 fuel from lignocellulosic or other feedstock. Specifically at least double mutant of filamentous fungi having the isoprenoid pathway results in production of trichodiene in commercial quantities. One embodiment of the invention relates to producing the fuel at the site of the lignocellulosic feedstock to reduce costs of shipping the feedstock.
- Inventors:
Hohn, Thomas (Chapel Hill, NC, US)
- Assignees:
NOVOZYMES A/S (Bagsvaerd, DK)
- Claim:
1-15. (canceled)
- Claim:
16. A method of producing trichodiene comprising: a) selecting a mutant filamentous fungus having the trichothecene pathway comprising: i. a disrupted Tri4 gene, a mutant Tri4 gene having low P450 monooxygenase production, and/or the presence of a Tri4 inhibitor sufficient to inhibit at least a portion of the Tri4 gene product; ii. a modified gene selected from Tri5, Tri6 and Tri10, the gene modified to increase the production of the gene product; b) cultivating the mutant filamentous fungus in a growth media culture; and c) isolating trichodiene from the growth media culture; wherein the mutant filamentous fungus produces at least 10% more trichodiene than the parent filamentous fungus when cultured under the same conditions.
- Claim:
17-18. (canceled)
- Claim:
19. The method according to claim 16 wherein the mutant filamentous fungus produces at least 0.25 g of trichodiene per gram of glucose or glucose equivalent consumed.
- Claim:
20. (canceled)
- Claim:
21. The method according to claim 16 wherein the mutant filamentous fungus is selected from the group consisting of Acremonium, Aspergillus, Aureobasidium, Cryptococcus, Filibasidium, Fusarium, Gibberella, Humicola, Magnaporthe, Mucor, Myceliophthora, Myrothecium, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Piromyces, Stachybotrys, Schizophyllum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Trichoderma, or Trichothecium strain.
- Claim:
22. The method according to claim 21 wherein the mutant filamentous fungus is Fusarium sporotrichioides.
- Claim:
23-25. (canceled)
- Claim:
26. The method according to claim 16 wherein the mutant filamentous fungus comprises a disrupted Tri4 gene, or a mutant Tri4 gene having low P450 monooxygenase production.
- Claim:
27. The method according to claim 16 wherein the mutant filamentous fungus comprises a nonrevertable site-selected deletion of part or all of nucleic acid encoding the Tri4 gene product such that the Tri4 gene is inactivated.
- Claim:
28. The method according to claim 16 wherein the Tri4 gene product enzymatic or catalytic activity is reduced by at least 10% when compared to the parent strain under the same conditions.
- Claim:
29. The method according to claim 16 wherein the mutant filamentous fungus is in the presence of a Tri4 inhibitor sufficient to inhibit at least a portion of the Tri4 gene product.
- Claim:
30. The method according to claim 16 wherein the modified gene has been modified to have constitutive activity in producing the gene product.
- Claim:
31. The method according to claim 16 wherein the mutant filamentous fungus comprises a modified Tri5 gene, the gene modified to increase the production of the gene product.
- Claim:
32. The method according to claim 16 wherein the mutant filamentous fungus comprises a modified Tri6 gene, the gene modified to increase the production of the gene product.
- Claim:
33. The method according to claim 16 wherein the mutant filamentous fungus comprises a modified Tri10 gene, the gene modified to increase the production of the gene product.
- Claim:
34. The method according to claim 16 wherein the mutant filamentous fungus comprises at least two modified genes selected from Tri5, Tri6, and Tri10, the genes modified to increase the production of the gene products.
- Claim:
35. The method according to claim 16 wherein the mutant filamentous fungus comprises a modified Tri5 gene, a modified Tri6 gene, and a modified Tri10 gene, the genes modified to increase the production of the gene products.
- Claim:
36. The method according to claim 16 wherein the modified gene comprises more than one copy of the nucleic acid sequence encoding for the gene product.
- Claim:
37. The method according to claim 36 wherein at least one of the additional copies of the nucleic acid sequence encoding for the gene product is in a vector which is capable of autonomous maintenance in the filamentous fungus.
- Claim:
38. The method according to claim 16 wherein the modified gene comprises a coding sequence operably linked to a promoter from a constitutively active filamentous fungal gene.
- Claim:
39. The method according to claim 16 wherein the mutant filamentous fungus is Fusarium.
- Claim:
40. The method according to claim 16 wherein the mutant filamentous fungus is Fusarium venenatam.
- Claim:
41. The method according to claim 16 wherein the mutant filamentous fungus is Fusarium gramineareum.
- Claim:
42. The method according to claim 16 wherein the growth media comprises growth media prepared from biomass.
- Claim:
43. The method according to claim 16 wherein the growth media comprises growth media prepared from lignocellulosic feedstock.
- Current U.S. Class:
435/166
- Current International Class:
12
- الرقم المعرف:
edspap.20120184008
Processing Request
No Comments.