PHYTOPHTHORA RESISTANCE IN SWEET PEPPERS

20120151622

12/963200

December 08, 2010

The present invention provides sweet bell pepper (Capsicum annuum) plants with high levels of resistance to Phytophthora combined with desirable agronomic traits. The present invention also provides methods of making such plants and methods of using such plants to produce additional Phytophthora-resistant sweet bell pepper plants.

Moreau, Michael (Saint Remy de Provence, FR); DENET, Frederic (Chateaurenard, FR); Sicard, Ophelie (Brain sur I'Authion, FR); Marchal, Cécile (St Remy de Provence, FR); Basterreix, Claude (Eygalieres, FR)

CLAUSE (Portes Les Valence, FR)

1. A Capsicum annuum plant resistant to Phytophthora, comprising quantitative Trait Loci (QTLs) associated with resistance to Phytophthora, wherein the QTLs map to linkage groups LG1/8, LG3, LG5, and LG10, and wherein said plant has a blocky type, a ¾ long type, or a half long type fruit shape.

2. The Capsicum annuum plant of claim 1, wherein the plant further comprises one or more QTLs associated with resistance to Phytophthora, wherein the QTLs map to a linkage group of LG2 and/or LG6.

3. The Capsicum annuum plant of claim 1, wherein the QTL from LG1/8 is genetically linked to molecular marker CAMS117 identified by amplification product of primers CAMS117-F and CAMS117-R, or linked to molecular marker CP10061 identified by amplification product of primers CP10061-F and CP 10061-R, wherein the QTL from LG3 is genetically linked to molecular marker CA517699 identified by amplification product of primers CA517699-F and CA517699-R, wherein the QTL from LG5 is genetically linked to molecular marker CAMS420 identified by amplification product of primers CAMS420-F and CAMS420-R, or linked to molecular marker CAMS190 identified by amplification product of primers CAMS190-F and CAMS190-R, or linked to molecular marker Epms749 identified by amplification product of primers Epms749-F and Epms749-R, and wherein the QTL from LG10 is genetically linked to molecular marker CAMS460 identified by amplification product of primers CAMS460-F and CAMS460-R.

4. The Capsicum annuum plant of claim 2, wherein the QTL from LG2 is genetically linked to molecular marker HpmsE090 identified by amplification product of primers HpmsE090-F and HpmsE090-R, or linked to molecular marker EPMS709 identified by amplification product of primers EPMS709-F and EPMS709-R, and wherein the QTL from LG6 is genetically linked to molecular marker AF208834 identified by amplification product of primers AF208834-F and AF208834-R.

5. The Capsicum annuum plant of claim 1, wherein the Phytophthora is P. capsici.

6. The Capsicum annuum plant of claim 1, wherein the plant has a level of resistance to P. capsici isolate ‘S197’ which is at least higher to the level of resistance of a susceptible check line selected from the group consisting of Alliance, Aristotle, Revolution.

7. The Capsicum annuum plant of claim 4, wherein the molecular marker AF208834 comprises about 228 nucleic acid base pairs.

8. The Capsicum annuum plant of claim 7, wherein the plant has a Phytophthora resistance score of 0 based on the stem decapitation test using P. capsici isolate ‘S197’ and a rating score of 0 to 5.

9. The Capsicum annuum plant of claim 4, wherein the molecular marker AF208834 comprises about 224 nucleic acid base pairs.

10. The Capsicum annuum plant of claim 9, wherein the plant has a Phytophthora resistance score of 1 or 2 based on the stem decapitation test using P. capsici isolate ‘S197’ and a rating score of 0 to 5.

11. The Capsicum annuum plant of claim 1, wherein the plant comprises one or more QTLs associated with fruit shape and/or fruit weight, wherein the QTLs associated with fruit shape and/or fruit weight map to linkage groups LG2, LG3, LG1/8, and/or LG10.

12. The Capsicum annuum plant of claim 11, wherein the QTL from LG2 associated with fruit shape and/or fruit weight is genetically linked to molecular marker PAPR2 identified by amplification product of primers PAPR2-F and PAPR2-R, wherein the QTL from LG3 associated with fruit shape and/or fruit weight is genetically linked to molecular marker EPMS648 identified by amplification product of primers EPMS648-F and EPMS648-R, wherein the QTL from LG1/8 associated with fruit shape and/or fruit weight is genetically linked to molecular marker HpmsMADS identified by amplification product of primers HpmsMADS-F and HpmsMADS-R, and wherein the QTL from LG10 associated with fruit shape and/or fruit weight is genetically linked to molecular marker GPMS159 identified by amplification product of primers GPMS159-F and GPMS159-R.

13. The Capsicum annuum plant of claim 12, wherein the molecular marker EPMS648 comprises about 188 nucleic acid base pairs and the plant has the ¾ long fruit shape.

14. The Capsicum annuum plant of claim 12, wherein the molecular marker EPMS648 comprises about 186 nucleic acid base pairs and the plant has blocky fruit shape.

15. A plant part obtained from the plant of claim 1 or claim 2.

16. The plant part of claim 15, wherein the plant part is a seed, a fruit, a scion, or a rootstock.

17. Seed of a Capsicum annuum line selected from the group consisting of ‘09SRTF713-5’, ‘09SRTF776-4’, and ‘09SRTF788-1’, wherein a representative sample of seed has been deposited under NCIMB No 41791, 41792 and 41793, respectively.

18. A plant, or part thereof, obtainable by growing the seed of claim 17.

19. A plant having all of the physiological and morphological characteristics of the plant of claim 18.

20. A tissue culture of regenerable cells produced from the plant, or part thereof, of claim 18.

21. An ovule of the plant of claim 18.

22. A pollen of the plant of claim 18.

23. A method for producing Capsicum annuum seed comprising crossing the plant of claim 18 with itself or another Capsicum annuum plant, and harvesting the resultant seed.

24. The seed of claim 17 or the resultant seed obtained of claim 23, wherein a plant grown from the seed comprises QTLs associated with resistance to Phytophthora, wherein the QTLs are genetically linked to molecular markers CAMS117, CAMS420, CA517699 and CAMS460 respectively, and wherein said plant having a blocky type, a % long type, or a half long type fruit shape.

25. A plant produced by germinating the seed of claim 24.

26. A seed produced by the plant of claim 25, wherein a plant produced by germinating said seed comprises QTLs associated with resistance to Phytophthora, wherein the QTLs are genetically linked to molecular markers CAMS117, CAMS420, CA517699 and CAMS460 respectively, and wherein said plant having a blocky type, a ¾ long type, or a half long type fruit shape.

27. A plant cell derived from the plant of claim 1.

28. A method for selecting the plant of claim 1, comprising the steps of: (i) detecting the presence of one or more QTLs associated with resistance to Phytophthora as indicated by the presence of one or more molecular markers selected from CAMS117, CP10061, CA517699, CAMS420, CAMS190, Epms749, CAMS460, wherein the QTLs are genetically linked to one of more said molecular markers; (ii) detecting the presence of one or more QTLs associated with fruit shape and/or fruit weight as indicated by the presence of one or more molecular markers selected from PAPR2, EPMS648, HpmsMADS, GPMS159, wherein the QTLs are genetically linked to one of more said molecular markers; (iii) selecting the plant comprising QTLs associated with resistance to Phytophthora from linkage groups LG1/8, LG3, LG5, and LG10, and QTLs associated with fruit shape and/or fruit weight from linkage groups LG2, LG3, LG1/8, and/or LG10; and (iv) optionally, confirming resistance to Phytophthora in the plant of step (iii) based on a stem decapitation test using P. capsici isolate ‘S197’, wherein the plant has a Phytophthora resistance score of 0 to 2.

29. The method of claim 28, wherein the molecular marker CAMS117 is defined by amplification product of primers CAMS117-F and CAMS117-R; the molecular marker CP10061 is defined by amplification product of primers CP10061-F and CP 10061-R; the molecular marker CA517699 is defined by amplification product of primers CA517699-F and CA517699-R; the molecular marker CAMS420 is defined by amplification product of primers CAMS420-F and CAMS420-R; the molecular marker CAMS190 is defined by amplification product of primers CAMS190-F and CAMS190-R; the molecular marker Epms749 is defined by amplification product of primers Epms749-F and Epms749-R; the molecular marker CAMS460 is defined by amplification product of primers CAMS460-F and CAMS460-R; and wherein the amplification product by using primers CAMS117-F and CAMS117-R, CP10061-F and CP 10061-R, CA517699-F and CA517699-R, CAMS420-F and CAMS420-R, CAMS190-F and CAMS190-R, Epms749-F and Epms749-R, or CAMS460-F and CAMS460-R from the Capsicum annuum plant resistant to Phytophthora is distinctive by size from an amplification product by using the same pair of primers from a check Capsicum annuum line, wherein the check Capsicum annuum line has a Phytophthora resistance score of 3 to 5 based on a stem decapitation test using P. capsici isolate ‘S197’.

30. The method of claim 28, wherein the method further comprises the steps of: (i) detecting the presence of one or more QTLs associated with resistance to Phytophthora as indicated by the presence of one or more molecular markers selected from HpmsE090, EPMS709, and AF208834, wherein the QTLs are genetically linked to one of more said molecular markers; (ii) selecting the plant comprising QTLs associated with resistance to Phytophthora from linkage groups LG2 and/or LG6.

31. The method of claim 30, wherein the molecular marker HpmsE090 is defined by amplification product of primers HpmsE090-F and HpmsE090-R; the molecular marker EPMS709 is defined by amplification product of primers EPMS709-F and EPMS709-R; the molecular marker AF208834 is defined by amplification product of primers AF208834-F and AF208834-R; and wherein the amplification product by using primers HpmsE090-F and HpmsE090-R, EPMS709-F and EPMS709-R, or AF208834-F and AF208834-R from the Capsicum annuum plant resistant to Phytophthora is distinctive by size from an amplification product by using the same pair of primers from a check Capsicum annuum line, wherein the check Capsicum annuum line has a Phytophthora resistance score of 3 to 5 based on a stem decapitation test using P. capsici isolate ‘S197’.

32. A progeny of the plant of claim 18 that comprises QTLs associated with resistance to Phytophthora, wherein the QTLs are genetically linked to molecular markers CAMS117, CAMS420, CA517699 and CAMS460 respectively, and wherein said plant has a blocky type, a ¾ long type, or a half long type fruit shape.

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