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NUCLEIC ACID MOLECULE

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اقرأ أكثر حفظ في قائمتي
  • Publication Date:
    August 4, 2011
  • معلومة اضافية
    • Document Number:
      20110190484
    • Appl. No:
      12/882927
    • Application Filed:
      September 15, 2010
    • نبذة مختصرة :
      The invention relates to an isolated nucleic acid molecule encoding a polypeptide capable of producing a triterpenoid hydrocarbon. The invention also relates to the encoded polypeptide, a vector comprising the nucleic acid molecule, a recombinant non-human organism comprising the nucleic acid molecule, and to methods of producing a triterpenoid hydrocarbon or an intermediate of biofuel using the nucleic acid molecule, polypeptide or recombinant organism.
    • Inventors:
      Ball, Andrew (Bedford Park SA, AU); Moore, Robert (Bedford Park SA, AU); Knowles, Gregory (Bedford Park SA, AU); Qin, Jian (Bedford Park SA, AU)
    • Assignees:
      WWCC Limited (Hong Kong, HK)
    • Claim:
      1. An isolated nucleic acid molecule encoding a polypeptide capable of producing a triterpenoid hydrocarbon, the polypeptide comprising SEQ ID NO: 29.
    • Claim:
      2. The nucleic acid molecule of claim 1, comprising SEQ ID NO: 30.
    • Claim:
      3-5. (canceled)
    • Claim:
      6. A recombinant non-human organism capable of producing a triterpenoid hydrocarbon, the organism comprising an isolated nucleic acid molecule encoding a polypeptide capable of producing a triterpenoid hydrocarbon, the polypeptide comprising SEQ ID NO: 29.
    • Claim:
      7. The recombinant organism of claim 6, wherein the organism is a microorganism.
    • Claim:
      8. The recombinant organism of claim 6, wherein the organism is an alga or a bacterium.
    • Claim:
      9. The recombinant organism of claim 6, wherein the organism is selected from the group consisting of: Escherichia coli; Chlamydomonas reinhardtii; Saccharomyces cerevisiae; and Pichia sp.
    • Claim:
      10. A method for producing a triterpenoid hydrocarbon, comprising expressing an isolated nucleic acid molecule encoding a polypeptide capable of producing a triterpenoid hydrocarbon, the polypeptide comprising SEQ ID NO: 29.
    • Claim:
      11-12. (canceled)
    • Claim:
      13. The method of claim 10, wherein the triterpenoid hydrocarbon is squalene.
    • Claim:
      14. The method of claim 10, wherein the triterpenoid hydrocarbon is dehydrosqualene.
    • Claim:
      15-19. (canceled)
    • Claim:
      20. The nucleic acid molecule of claim 1, wherein the triterpenoid hydrocarbon is produced from FPP or PSPP.
    • Claim:
      21. The nucleic acid molecule or claim 1, wherein the triterpenoid hydrocarbon is squalene.
    • Claim:
      22. (canceled)
    • Claim:
      23. The recombinant organism of claim 6, wherein the nucleic acid molecule comprises SEQ ID NO: 30.
    • Claim:
      24. The recombinant organism of claim 6, wherein the triterpenoid hydrocarbon is produced from FPP or PSPP.
    • Claim:
      25. The recombinant organism of claim 6, wherein the triterpenoid hydrocarbon is squalene.
    • Claim:
      26. The recombinant organism of claim 6, wherein the triterpenoid hydrocarbon is dehydrosqualene.
    • Claim:
      27. The method of claim 10, wherein the nucleic acid molecule comprises SEQ ID NO: 30.
    • Claim:
      28. The method of claim 10, wherein the triterpenoid hydrocarbon is produced from FPP or PSPP.
    • Claim:
      29. The nucleic acid molecule of claim 1, wherein the triterpenoid hydrocarbon is dehydrosqualene.
    • Current U.S. Class:
      536/232
    • Current International Class:
      07; 12; 12; 12; 12; 12; 12; 12; 07; 07
    • الرقم المعرف:
      edspap.20110190484