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Fully automated 18F-fluorination of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB) for indirect labelling of nanobodies.

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  • معلومة اضافية
    • Contributors:
      GIGA CRC (Cyclotron Research Center) In vivo Imaging-Aging & Memory - ULiège
    • بيانات النشر:
      Springer Science and Business Media LLC, 2022.
    • الموضوع:
      2022
    • نبذة مختصرة :
      N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB), a widely used labeling agent to introduce the 4-[18F]fluorobenzoyl-prosthetic group, is normally obtained in three consecutive steps from [18F]fluoride ion. Here, we describe an efficient one-step labeling procedure of [18F]SFB starting from a tin precursor. This method circumvents volatile radioactive side-products and simplifies automatization. [18F]SFB was obtained after HPLC purification in a yield of 42 + 4% and a radiochemical purity (RCP) > 99% (n = 6). In addition, we investigate the automation of the coupling of [18F]SFB to a nanobody (cAbBcII10, targeting β-lactamase enzyme) and purification by size exclusion chromatography (PD-10 desalting column) to remove unconjugated reagent. Production and use of [18F]SFB were implemented on a radiosynthesis unit (Neptis®). The fully automated radiosynthesis process including purification and formulation required 160 min of synthesis time. [18F]SFB-labeled nanobody was obtained in a yield of 21 + 2% (activity yield 12 + 1% non-decay corrected) and a radiochemical purity (RCP) of > 95% (n = 3). This approach simplifies [18F]SFB synthesis to one-step, enhances the yield in comparison to the previous report and enables the production of radiolabeled nanobody on the same synthesis module.
      Biowin Program PIT of the Walloon Region
    • Relation:
      https://www.nature.com/articles/s41598-022-23552-8.pdf; urn:issn:2045-2322
    • الرقم المعرف:
      10.1038/s41598-022-23552-8
    • Rights:
      open access
      http://purl.org/coar/access_right/c_abf2
      info:eu-repo/semantics/openAccess
    • الرقم المعرف:
      edsorb.296341