Detecting RNA G-Quadruplexes (rG4s) in the Transcriptome.

Cold Spring Harbor Laboratory Press https://doi.org/10.1101/cshperspect.a032284 Cold Spring Harbor Perspectives in Biology 2018-10-03T04:43:09Z 2018-10-03T04:43:09Z 2018-07-02

SUMMARY RNA G-quadruplex (rG4) secondary structures are proposed to play key roles in fundamental biological processes that include the modulation of transcriptional, co-transcriptional, and posttranscriptional events. Recent methodological developments that include predictive algorithms and structure-based sequencing have enabled the detection and mapping of rG4 structures on a transcriptome-wide scale at high sensitivity and resolution. The data generated by these studies provide valuable insights into the potentially diverse roles of rG4s in biology and open up a number of mechanistic hypotheses. Herein we highlight these methodologies and discuss the associated findings in relation to rG4-related biological mechanisms.
The Balasubramanian laboratory is supported by European Research Council Advanced Grant No. 339778, a Wellcome Trust Senior Investigator Award, and core funding from Cancer Research UK. The Kwok laboratory is supported by City University of Hong Kong Project No. 9610363, 7200520, Croucher Foundation Project No. 9500030, and Hong Kong RGC Project No. CityU 21302317, N_CityU110/17.

Algorithms; G-Quadruplexes; Humans; RNA; Sensitivity and Specificity; Transcriptome; Article

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HS1 oai:www.repository.cam.ac.uk:1810/283013
10.17863/CAM.30377
1489043934

UNIV OF CAMBRIDGE
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