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Genetically targeted 3D visualisation of $\textit{Drosophila}$ neurons under Electron Microscopy and X-Ray Microscopy using miniSOG

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  • معلومة اضافية
    • Publisher Information:
      Nature Publishing Group https://doi.org/10.1038/srep38863 Scientific Reports 2017-01-31T15:39:48Z 2017-01-31T15:39:48Z 2016-12
    • نبذة مختصرة :
      Large dimension, high-resolution imaging is important for neural circuit visualisation as neurons have both long- and short-range patterns: from axons and dendrites to the numerous synapses at terminal endings. Electron Microscopy (EM) is the favoured approach for synaptic resolution imaging but how such structures can be segmented from high-density images within large volume datasets remains challenging. Fluorescent probes are widely used to localise synapses, identify cell-types and in tracing studies. The equivalent EM approach would benefit visualising such labelled structures from within sub-cellular, cellular, tissue and neuroanatomical contexts. Here we developed genetically-encoded, electron-dense markers using miniSOG. We demonstrate their ability in 1) labelling cellular sub-compartments of genetically-targeted neurons, 2) generating contrast under different EM modalities, and 3) segmenting labelled structures from EM volumes using computer-assisted strategies. We also tested non-destructive X-ray imaging on whole Drosophila brains to evaluate contrast staining. This enabled us to target specific regions for EM volume acquisition.
      This work was supported by European Research Council Starting Investigator and Consolidator grants, the European Molecular Biology Organization (EMBO) Young Investigator Programme, and Medical Research Council (MRC) Grant MC-U105188491 (G.S.X.E.J).
    • الموضوع:
      Animals; Animals, Genetically Modified; Axons; Dendrites; Drosophila; Image Enhancement; Imaging, Three-Dimensional; Microscopy, Electron; Neurons; X-Rays; Article
    • Availability:
      Open access content. Open access content
      Attribution 4.0 International
      Attribution 4.0 International
      Attribution 4.0 International
      http://creativecommons.org/licenses/by/4.0
      http://creativecommons.org/licenses/by/4.0
      http://creativecommons.org/licenses/by/4.0
    • Note:
      application/pdf
      application/pdf
      English
      English
    • Other Numbers:
      HS1 oai:www.repository.cam.ac.uk:1810/262199
      10.17863/CAM.7456
      1488178280
    • Contributing Source:
      UNIV OF CAMBRIDGE
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1488178280
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