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SWR1 chromatin remodeling complex prevents mitotic slippage during spindle position checkpoint arrest

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  • معلومة اضافية
    • Publisher Information:
      American Society for Cell Biology International 2023
    • نبذة مختصرة :
      Faithful chromosome segregation in budding yeast requires correct positioning of the mitotic spindle along the mother to daughter cell polarity axis. When the anaphase spindle is not correctly positioned, a surveillance mechanism, named as the spindle position checkpoint (SPOC), prevents the progression out of mitosis until correct spindle positioning is achieved. How SPOC works on a molecular level is not well understood. Here we performed a genome-wide genetic screen to search for components required for SPOC. We identified the SWR1 chromatin-remodeling complex (SWR1-C) among several novel factors that are essential for SPOC integrity. Cells lacking SWR1-C were able to activate SPOC upon spindle misorientation but underwent mitotic slippage upon prolonged SPOC arrest. This mitotic slippage required the Cdc14-early anaphase release pathway and other factors including the SAGA (Spt-Ada-Gcn5 acetyltransferase) histone acetyltransferase complex, proteasome components and the mitotic cyclin-dependent kinase inhibitor Sic1. Together, our data establish a novel link between SWR1-C chromatin remodeling and robust checkpoint arrest in late anaphase.
      We thank Astrid Hofmann and Dorothee Albrecht for excellent technical assistance and Elmar Schiebel for strains and access to microscopes. This work was funded by the Deutsche Forschungsgemein-schaft (DFG) Collaborative Research Center SFB1036 (Project TP21 to G.P. and Project TP10 to M.K. and A.K.). A.K.C. was supported by the German Research Council (DFG, PE1883/1-2), MSCA Individual Fellowship (796599, COHEMEX), and EMBO-IG (3918). The work of G.P. is supported by the Heisenberg program (PE1883/3), SFB873/A14 and SFB1324/B09 of the DFG.
    • الموضوع:
      Cell biology; SWR1 chromatin; Spindle position checkpoint (SPOC); Journal article; text/academic publication
    • Availability:
      Open access content. Open access content
    • Note:
      pdf
      English
    • Other Numbers:
      T9K oai:libdigitalcollections.ku.edu.tr:IR/11228
      IR04345
      1059-1524
      1939-4586
      Caydasi, Ayse Koca, et al. “Swr1 Chromatin Remodeling Complex Prevents Mitotic Slippage during Spindle Position Checkpoint Arrest.” Molecular Biology of the Cell, vol. 34, no. 2, 2023, p. 11, https://doi.org/10.1091/mbc.E20-03-0179.
      WoS; Scopus; Pubmed
      NA
      NA
      1429869382
    • Contributing Source:
      KOC UNIV LIBR
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1429869382
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