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Sound waves for solving the problem of recrystallization in cryopreservation

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  • معلومة اضافية
    • Publisher Information:
      Springer 2023
    • نبذة مختصرة :
      Organ biobanking is the pending subject of cryopreservation. Although the problem is multifaceted, advances in recent decades have largely related it to achieving rapid and uniform rewarming of cryopreserved samples. This is a physical challenge largely investigated in past in addition to cryoprotectant toxicity studies, which have also shown a great amount of advancement. This paper presents a proof-of-principle, based on the nematode Caenorhabditis elegans, of a technology capable of performing such a function: high intensity focused ultrasound. Thus, avoiding the problem of recrystallization, this worm, in its adult state, preserved at − 80∘C , has been systematically brought back to life after being heated with High Intensity Focused Ultrasound (HIFU) waves. The great advantage of this technology is that it is scalable; in addition, rewarming can be monitored in real time by MRI thermography and can be controlled by acoustic interferometry. We anticipate that our findings are the starting point for a possible approach to rewarming that can be used for cryopreservation of millimeter scale systems: either alone or in combination with other promising ways of heating, like nanowarming or dielectric heating, the present technology provides new ways of solving the physical aspects of the problem of recrystallization in cryopreservation, opening the door for the long-term storage of larger samples.
    • الموضوع:
      info:eu-repo/semantics/article
    • Availability:
      Open access content. Open access content
      http://creativecommons.org/licenses/by/4.0
      info:eu-repo/semantics/openAccess
      Atribución 4.0 Internacional
    • Note:
      English
    • Other Numbers:
      SUE oai:idus.us.es:11441/148388
      Alcalá Guerrero, E., Encabo, L., Barroso, F., Puentes González-Amor, A., Risco, I. y Risco, R. (2023). Sound waves for solving the problem of recrystallization in cryopreservation. Scientific Reports, 13 (1). https://doi.org/10.1038/s41598-023-34681-z.
      2045-2322
      10.1038/s41598-023-34681-z
      1416310066
    • Contributing Source:
      UNIV DE SEVILLA
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1416310066
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