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Identification of a new Kir6 potassium channel and comparison of properties of Kir6 subtypes by structural modelling and molecular dynamics

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  • معلومة اضافية
    • Publisher Information:
      Uppsala universitet, Science for Life Laboratory, SciLifeLab Uppsala universitet, Institutionen för medicinsk cellbiologi Univ Nottingham, Sch Chem, Nottingham NG7 2RD, England. Nicolaus Copernicus Univ Torun, Inst Phys, Fac Phys Astron & Informat, Ul Grudziadzka 5, PL-87100 Torun, Poland. Elsevier BV 2023
    • نبذة مختصرة :
      ATP-sensitive potassium ion channels (KATP) are transmembrane proteins that modulate insulin release and muscle contraction. KATP channels are composed of two types of subunit, Kir6 and SUR, which exist in two and three isoforms respectively with different tissue distribution. In this work, we identify a previously undescribed ancestral vertebrate gene encoding a Kir6-related protein that we have named Kir6.3, which may not have a SUR binding partner, unlike the other two Kir6 proteins. Whereas Kir6.3 was lost in amniotes including mammals, it is still present in several early-diverging vertebrate lineages such as frogs, coelacanth, and rayfinned fishes. Molecular dynamics (MD) simulations using homology models of Kir6.1, Kir6.2, and Kir6.3 from the coelacanth Latimeria chalumnae showed that the three proteins exhibit subtle differences in their dynamics. Steered MD simulations of Kir6-SUR pairs suggest that Kir6.3 has a lower binding affinity for the SUR proteins than either Kir6.1 or Kir6.2. As we found no additional SUR gene in the genomes of the species that have Kir6.3, it most likely forms a lone tetramer. These findings invite studies of the tissue distribution of Kir6.3 in relation to the other Kir6 as well as SUR proteins to determine the functional roles of Kir6.3.
    • الموضوع:
      ATP sensitive potassium channel; Molecular dynamics; Gene family and evolution; Physiology and Anatomy; Fysiologi och anatomi; Article in journal; info:eu-repo/semantics/article; text
    • الرقم المعرف:
      10.1016.j.ijbiomac.2023.125771
    • Availability:
      Open access content. Open access content
      info:eu-repo/semantics/openAccess
    • Note:
      application/pdf
      English
    • Other Numbers:
      UPE oai:DiVA.org:uu-509280
      0000-0002-6736-0663
      doi:10.1016/j.ijbiomac.2023.125771
      PMID 37433419
      ISI:001040000800001
      1399993114
    • Contributing Source:
      UPPSALA UNIV LIBR
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1399993114
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