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Response regulator PorX coordinates oligonucleotide signalling and gene expression to control the secretion of virulence factors
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- المؤلفون: National Science Centre (Poland); Ministerio de Ciencia, Innovación y Universidades (España); Agencia Estatal de Investigación (España); Ministerio de Economía y Competitividad (España); Generalitat de Catalunya; National Institute of Dental and Craniofacial Research (US); Ministry of Science and Higher Education (Poland); Wroclaw Center for Biotechnology; Schmitz, Claus A.; Madej, Mariusz; Nowakowska, Zuzanna; Cuppari, Anna; Jacula, Anna; Ksiazek, Miroslaw; Mikruta, Katarzyna; Wisniewski, Jerzy; Pudelko-Malik, Natalia; Saran, Anshu; Zeytuni, Natalie; Mlynarz, Piotr; Lamont, Richard J; Usón, Isabel; Siksnys, Virginijus; Potempa, Jan; Sola, María
- نوع التسجيلة:
Electronic Resource
- الدخول الالكتروني :
http://hdl.handle.net/10261/305713
Publisher's version
The underlying dataset has been published as supplementary material of the article in the publisher platform at 10.1093/nar/gkac1103
http://dx.doi.org/10.1093/nar/gkac1103
Sí
info:eu-repo/grantAgreement/MINECO//BFU2015-70645-R/ES/ANALISIS ESTRUCTURAL DEL GENOMA MITOCONDRIAL Y SU REGULACION
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-101015-B-I00/ES/ANALISIS ESTRUCTURAL DE PROTEINAS ESENCIALES PARA LA VIABILIDAD MITOCONDRIAL Y DE PATOGENOS
info:eu-repo/grantAgreement/AEI//PID2021-129038NB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-101370-B-I00/ES/DESARROLLO DE METODOS CRISTALOGRAFICOS (VI) Y SU APLICACION A CATALISIS AVANZADA
info:eu-repo/grantAgreement/AEI//PID2021-128751NB-I00
- معلومة اضافية
- Publisher Information:
Oxford University Press 2022-11-28
- نبذة مختصرة :
The PglZ family of proteins belongs to the alkaline phosphatase superfamily, which consists of metallohydrolases with limited sequence identity but similar metal-coordination architectures in otherwise divergent active sites. Proteins with a well-defined PglZ domain are ubiquitous among prokaryotes as essential components of BREX phage defence systems and two-component systems (TCSs). Whereas other members of the alkaline phosphatase superfamily are well characterized, the activity, structure and biological function of PglZ family proteins remain unclear. We therefore investigated the structure and function of PorX, an orphan response regulator of the Porphyromonas gingivalis TCS containing a putative PglZ effector domain. The crystal structure of PorX revealed a canonical receiver domain, a helical bundle, and an unprecedented PglZ domain, similar to the general organization of the phylogenetically related BREX-PglZ proteins. The PglZ domain of PorX features an active site cleft suitable for large substrates. An extensive search for substrates revealed that PorX is a phosphodiesterase that acts on cyclic and linear oligonucleotides, including signalling molecules such as cyclic oligoadenylates. These results, combined with mutagenesis, biophysical and enzymatic analysis, suggest that PorX coordinates oligonucleotide signalling pathways and indirectly regulates gene expression to control the secretion of virulence factors.
- الموضوع:
- Availability:
Open access content. Open access content
https://creativecommons.org/licenses/by-nc/4.0
openAccess
- Other Numbers:
CTK oai:digital.csic.es:10261/305713
Nucleic Acids Research 50(21): 12558-12577 (2022)
10.1093/nar/gkac1103
1380453489
- Contributing Source:
CSIC
From OAIster®, provided by the OCLC Cooperative.
- الرقم المعرف:
edsoai.on1380453489
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