Glycomic analysis of the in vitro modification and utilization of chicken intestinal mucin O-glycan carbohydrates by the enteric poultry pathogen Clostridium perfringens

Lethbridge, Alta. : Universtiy of Lethbridge, Department of Biological Sciences Department of Biological Sciences Arts and Science 2019

Clostridium perfringens is the etiological agent of necrotic enteritis (NE) in poultry. NE occurs within the small intestine and is characterized by intestinal mucus depletion and tissue inflammation. This disease costs the global poultry industry an estimated USD $2-6 billion per year. The small intestine mucus glycans of birds contain a large abundance of sialic acid (Neu5Ac) and sulfate, which may impede colonization by enteric avian bacterial pathogens. C. perfringens was shown here to liberate and metabolize Neu5Ac from extracted broiler chicken mucin O-glycans. By correlating these findings with carbohydrate active enzymes active on mucin O-glycans and the core metabolic enzymes present within C. perfringens, a biochemical basis for these relationships can be established. These new findings suggest that Neu5Ac utilization is significant for mucus colonization by C. perfringens and may provide C. perfringens with a competitive advantage in the avian intestine

Mucin; Carbohydrate; Glycan; Intestinal Mucus; Clostridium perfringens; Broiler chicken; Porcine gastric mucin; Dissertations, Academic; Thesis

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CN4UL oai:opus.uleth.ca:10133/5532
1375492606

UNIV OF LETHBRIDGE
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