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Pih1p-Tah1p puts a lid on hexameric AAA+ ATPases Rvb1/2p

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  • معلومة اضافية
    • Publisher Information:
      Cell Press Department of Chemistry and Biochemistry Arts and Science Florida State University University of Texas Health Science Center at San Antonio University of Lethbridge http://dx.doi.org/10.1016/j.str.2017.08.002 2017
    • نبذة مختصرة :
      The Saccharomyces cerevisiae (Sc) R2TP complex affords an Hsp90-mediated and nucleotide-driven chaperone activity to proteins of small ribonucleoprotein particles (snoRNPs). The current lack of structural information on the ScR2TP complex, however, prevents a mechanistic understanding of this biological process. We characterized the structure of the ScR2TP complex made up of two AAA+ ATPases, Rvb1/2p, and two Hsp90 binding proteins, Tah1p and Pih1p, and its interaction with the snoRNP protein Nop58p by a combination of analytical ultracentrifugation, isothermal titration calorimetry, chemical crosslinking, hydrogen-deuterium exchange, and cryoelectron microscopy methods. We find that Pih1p-Tah1p interacts with Rvb1/2p cooperatively through the nucleotide-sensitive domain of Rvb1/2p. Nop58p further binds Pih1p-Tahp1 on top of the dome-shaped R2TP. Consequently, nucleotide binding releases Pih1p-Tah1p from Rvb1/2p, which offers a mechanism for nucleotide-driven binding and release of snoRNP intermediates.
    • الموضوع:
    • Availability:
      Open access content. Open access content
    • Note:
      en_US
    • Other Numbers:
      CN4UL oai:opus.uleth.ca:10133/5998
      Tian, S., Yu, G., He, H., Zhao, Y., Liu, P., Marshall, A. G., Demeler, B., Stagg, S. M., & Li, H. (2017). Pih1p-Tah1p puts a lid on hexameric AAA+ ATPases Rvb1/2p. Structure, 25(10), 1519-1529. http://dx.doi.org/10.1016/j.str.2017.08.002
      1375487062
    • Contributing Source:
      UNIV OF LETHBRIDGE
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1375487062
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