Nuclear microsatellites for Pinus pinea (Pinaceae), a genetically depauperate tree, and their transferability to P. halepensis

John Wiley & Sons 2012

• Premise of the study Pinus pinea is one of the few widespread plant species that are also genetically depauperate. It is also an important commercial species with high market value seeds. A deeper knowledge of the existing population genetic variation was needed. • Methods and Results Twelve nuclear microsatellites were isolated from genomic and cDNA sequences and screened for variability in 729 individuals from 33 natural populations. Low level of genetic variability was confirmed with average expected heterozygosity of 0.11. Hardy-Weinberg equilibrium expectations were not met in only ~10% of the possible locus/population combinations. All loci were in linkage equilibrium, and the frequency of null alleles was very low (≤ 1% in 332 out of 396 locus/ population combinations). Nine out of the 12 microsatellites were successfully transferred to P. halepensis. • Conclusions Despite low polymorphism, these new markers will be useful to resolve population structure and hold potential for seed origin identification and traceability. © 2012 Botanical Society of America.

Genetic diversity; Microsatellites; Population genetics; Pinaceae; Pinus halepensis; Pinus pinea; artículo

Open access content. Open access content
closedAccess

English

CTK oai:digital.csic.es:10261/292831
American Journal of 99(9): 362-365 (2012)
0002-9122
10.3732/ajb.1200064
1537-2197
1373155533

CSIC
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