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Qualitative HPLC-DAD/ESI-TOF-MS Analysis, Cytotoxic, and Apoptotic Effects of Croatian Endemic Centaurea ragusina L. Aqueous Extracts

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  • معلومة اضافية
    • Publisher Information:
      Wiley-V C H Verlag Gmbh, Weinheim 2017
    • نبذة مختصرة :
      Centaurea ragusina L., an endemic Croatian plant species, revealed a good cytotoxic activity of aqueous extracts (AE) on human bladder (T24) and human glioblastoma (A1235) cancer cell lines. The chemical constituents were tentatively identified using high performance liquid chromatography HPLC-DAD/ ESI-TOF-MS in negative ionization mode. The main compounds of herba extract were sesquiterpene lactones: solstitialin A 3,13-diacetate and epoxyrepdiolide; organic acid: quinic acid. The main compounds of flower extract were organic acids: quinic acid, citric acid, and malic acid; sesquiterpene lactone: cynaropicrin; phenolic compounds: chlorogenic acid and phenylpropanoid: syringin. The AE of C. ragusina were investigated for correlation of their effects on human bladder (T24) and human glioblastoma (A1235) cancer cell lines using the MTT assay. Although both extracts showed significant dose-and time-dependent cytotoxic activity against both cancer cell lines, the flower extract exhibited slightly higher activity. In order to determine type of cell death induced by treatment, cell lines were exposed subsequently to a treatment with both flower and herba AE. The majority of the cells died by induced apoptosis treatment. Flower AE (26.25%), compared to a leaf AE (22.15%) showed slightly higher percentage of an apoptosis in T24 cells, when compared to a non-treated cells (0.04%).
    • الموضوع:
      Apoptotic activities; Centaurea ragusina; Cytotoxic activities; MTT; Sesquiterpene lactone; article
    • Availability:
      Open access content. Open access content
      ARR
      embargoedAccess
    • Note:
      Chemistry and Biodiversity
    • Other Numbers:
      RSPST oai:cherry.chem.bg.ac.rs:123456789/3245
      1612-1872
      https://cherry.chem.bg.ac.rs/handle/123456789/3245
      000410558700004
      10.1002/cbdv.201700099
      2-s2.0-85026497390
      https://cherry.chem.bg.ac.rs/bitstream/id/8082/radan2017.pdf
      1365509300
    • Contributing Source:
      UIVERSITY OF BELGRADE
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1365509300
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