Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Distributed interfacing by nanoscale photodiodes enables single-neuron light activation and sensory enhancement in 3D spinal explants

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • Publisher Information:
      American Association for the Advancement of Science 2022-08
    • نبذة مختصرة :
      Among emerging technologies developed to interface neuronal signaling, engineering electrodes at the nanoscale would yield more precise biodevices opening to progress in neural circuit investigations and to new therapeutic potential. Despite remarkable progress in miniature electronics for less invasive neurostimulation, most nano-enabled, optically triggered interfaces are demonstrated in cultured cells, which precludes the studies of natural neural circuits. We exploit here free-standing silicon-based nanoscale photodiodes to optically modulate single, identified neurons in mammalian spinal cord explants. With near-infrared light stimulation, we show that activating single excitatory or inhibitory neurons differently affects sensory circuits processing in the dorsal horn. We successfully functionalize nano-photodiodes to target single molecules, such as glutamate AMPA receptor subunits, thus enabling light activation of specific synaptic pathways. We conclude that nano-enabled neural interfaces can modulate selected sensory networks with low invasiveness. The use of nanoscale photodiodes can thus provide original perspective in linking neural activity to specific behavioral outcome.
    • الموضوع:
      intrinsic silicon; dorsal horn; pain; receptor; circuits; interneurons; mechanisms; expression; patterns; cultures; info:eu-repo/semantics/article
    • Availability:
      Open access content. Open access content
    • Note:
      We thank M. Gigante, G. Cellot, S. Mortal, and M. Lazzarino for help with setting up the laser stimulation setup; the group of P. Heppenstall for help with the AAV production; M. Giugliano for MATLAB code optimization; R. Owen for suggestions in antibody nPD characterization; and S. Usmani for help with initial experiments. This project was funded by HFSP program grant RGP0004/2019 (to L.B., B.T., and L.F.).
      English
    • Other Numbers:
      ESUPV oai:addi.ehu.eus:10810/58282
      1364683062
    • Contributing Source:
      REPOSITORIO DE LA UNIVERSIDAD DEL PAIS
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1364683062
HoldingsOnline