Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

IL-1beta-induced chemokine and Fas expression are inhibited by suppressor of cytokine signalling-3 in insulin-producing cells.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
اقرأ أكثر حفظ في قائمتي
  • المؤلفون: Jacobsen, M L B; Rønn, S G; Bruun, C; Larsen, C M; Eizirik, Decio L.; Mandrup-Poulsen, T.; Billestrup, N
  • المصدر:
    Diabetologia, 52 (2
  • نوع التسجيلة:
    Electronic Resource
  • الدخول الالكتروني :
    https://dipot.ulb.ac.be/dspace/bitstream/2013/52506/3/doi_27720.pdf
    http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/52506
    https://worldcat.org/search?q=on:EQY+http://difusion-oai.ulb.ac.be/oai/request+DCG_ENTIRE_REPOSITORY+CNTCOLL
  • معلومة اضافية
    • Publisher Information:
      2009
    • نبذة مختصرة :
      AIMS/HYPOTHESIS: Chemokines recruit activated immune cells to sites of inflammation and are important mediators of insulitis. Activation of the pro-apoptotic receptor Fas leads to apoptosis-mediated death of the Fas-expressing cell. The pro-inflammatory cytokines IL-1beta and IFN-gamma regulate the transcription of genes encoding the Fas receptor and several chemokines. We have previously shown that suppressor of cytokine signalling (SOCS)-3 inhibits IL-1beta- and IFN-gamma-induced nitric oxide production in a beta cell line. The aim of this study was to investigate whether SOCS-3 can influence cytokine-induced Fas and chemokine expression in beta cells. METHODS: Using a beta cell line with inducible Socs3 expression or primary neonatal rat islet cells transduced with a Socs3-encoding adenovirus, we employed real-time RT-PCR analysis to investigate whether SOCS-3 affects cytokine-induced chemokine and Fas mRNA expression. The ability of SOCS-3 to influence the activity of cytokine-responsive Fas and Mcp-1 (also known as Ccl2) promoters was measured by reporter analysis. RESULTS: IL-1beta induced a time-dependent increase in Mcp-1 and Mip-2 (also known as Cxcl2) mRNA expression after 6 h of stimulation in insulinoma (INS)-1 and neonatal rat islet cells. This induction was inhibited when Socs3 was expressed in the cells. In INS-1 cells, IL-1beta + IFN-gamma induced a tenfold and eightfold increase of Fas mRNA expression after 6 and 24 h, respectively. This induction was inhibited at both time-points when expression of Socs3 was induced. In promoter studies SOCS-3 significantly inhibited the cytokine-induced activity of Mcp-1 and Fas promoter constructs. CONCLUSIONS/INTERPRETATION: SOCS-3 inhibits the expression of cytokine-induced chemokine and death-receptor Fas mRNA.
      Journal Article
      Research Support, Non-U.S. Gov't
      info:eu-repo/semantics/published
    • الموضوع:
      Sciences bio-médicales et agricoles; Animals; Cell Line; Chemokine CCL2 -- genetics; Chemokines -- genetics; Insulin -- biosynthesis; Insulin-Secreting Cells -- drug effects; Insulin-Secreting Cells -- physiology; Interferon-gamma -- pharmacology; Interleukin-1beta -- pharmacology; RNA, Messenger -- genetics; Rats; Signal Transduction; Suppressor of Cytokine Signaling Proteins -- physiology; Transcription, Genetic -- drug effects; Autoimmunity; Chemokine; Diabetes; IL-1; Inflammation; NF-kappaB; Signalling; SOCS; info:eu-repo/semantics/article; info:ulb-repo/semantics/articlePeerReview; info:ulb-repo/semantics/openurl/article
    • Availability:
      Open access content. Open access content
      1 full-text file(s): info:eu-repo/semantics/restrictedAccess
    • Note:
      1 full-text file(s): application/pdf
      English
    • Other Numbers:
      EQY oai:dipot.ulb.ac.be:2013/52506
      uri/info:doi/10.1007/s00125-008-1199-1
      uri/info:pmid/19002429
      uri/info:scp/58149456481
      1363711955
    • Contributing Source:
      UNIVERSITE LIBRE DE BRUXELLES
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1363711955
HoldingsOnline