Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription.

Rockefeller University Press 1991-03-01

Virus-transformed pre-B cells undergo ordered immunoglobulin (Ig) gene rearrangements during culture. We devised a series of highly sensitive polymerase chain reaction assays for Ig gene rearrangement and unrearranged Ig gene segment transcription to study both the possible relationship between these processes in cultured pre-B cells and the role played by heavy (H) chain (mu) protein in regulating gene rearrangement. Our analysis of pre-B cell cultures representing various stages of maturity revealed that transcription of each germline Ig locus precedes or is coincident with its rearrangement. Cell lines containing one functional rearranged H chain allele, however, continue to transcribe and to rearrange the allelic, unrearranged H chain locus. These cell lines appear to initiate but not terminate rearrangement events and therefore provide information about the requirements for activating rearrangement but not about allelic exclusion mechanisms.

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CC BY-NC-SA
https://creativecommons.org/licenses/by-nc-sa/4.0

UMV oai:jupiter.its.unimelb.edu.au:11343/259117
Schlissel, M. S., Corcoran, L. M. & Baltimore, D. (1991). Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription.. J Exp Med, 173 (3), pp.711-720. https://doi.org/10.1084/jem.173.3.711.
10.1084/jem.173.3.711
1540-9538
0022-1007
1315701924

UNIV OF MELBOURNE
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