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Retinal transplants restore visual responses: trans-synaptic tracing from visually responsive sites labels transplant neurons.

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اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • Publisher Information:
      eScholarship, University of California 2008-07-01
    • نبذة مختصرة :
      This study aimed to test the hypothesis that visual responses in the superior colliculus (SC) originate from synaptic connections between fetal retinal transplants and degenerating host retinas. Sheets of embryonic day 19 rat retina expressing human placental alkaline phosphatase were transplanted to the subretinal space of 3- to 4-week-old S334ter-line-3 rats with fast retinal degeneration. Several months later, visual responses were recorded from the SC. Attenuated pseudorabies virus that is specifically transferred between neurons at synapses (strains PRV-152, expressing green fluorescent protein (GFP) or BaBlu, expressing Escherichia colibeta-galactosidase) was injected into the visually responsive site of the SC. After survival times of 1-2 days, the virus was detected in the retina by immunohistochemistry in combination with different retinal cell markers, such as protein kinase C, recoverin, calcium-calmodulin-dependent protein kinase II and glutamine synthetase. Transplanted rats had a mean response threshold of -3.1 log cd/m(2) in a small area of the SC corresponding to the location of the graft in the retina. By 30 h after injection into this SC area, the virus traced back to host ganglion cells overlying the transplant and in close proximity to the transplant. By 2 days after injection, extensive virus label was found in the host retina and many cells in the transplant were also labeled. Virus-labeled cells in the transplant were double labeled for neuronal and glial cell markers. This study provides anatomical evidence that synaptic connections between fetal retinal transplants and host retinas contribute to the visual responses in the SC.
    • الموضوع:
      Visual Pathways; Neurons; Synapses; Retina; Animals; Genetically Modified; Humans; Rats; Herpesvirus 1; Suid; Fetal Tissue Transplantation; Staining and Labeling; Visual Perception; Superior Colliculi; Vision; Ocular; Biomarkers; Neurology & Neurosurgery; Neurosciences; Cognitive Sciences; Psychology; article
    • Note:
      application/pdf
      The European journal of neuroscience vol 28, iss 1, 208-220 0953-816X
    • Other Numbers:
      CDLER oai:escholarship.org:ark:/13030/qt7d9054n7
      qt7d9054n7
      https://escholarship.org/uc/item/7d9054n7
      https://escholarship.org/
      1287428738
    • Contributing Source:
      UC MASS DIGITIZATION
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1287428738
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