The cooperative assembly of shelterin bridge provides a kinetic gateway that controls telomere length homeostasis.

eScholarship, University of California 2021-08-01

Shelterin is a six-protein complex that coats chromosome ends to ensure their proper protection and maintenance. Similar to the human shelterin, fission yeast shelterin is composed of telomeric double- and single-stranded DNA-binding proteins, Taz1 and Pot1, respectively, bridged by Rap1, Poz1 and Tpz1. The assembly of the proteinaceous Tpz1-Poz1-Rap1 complex occurs cooperatively and disruption of this shelterin bridge leads to unregulated telomere elongation. However, how this biophysical property of bridge assembly is integrated into shelterin function is not known. Here, utilizing synthetic bridges with a range of binding properties, we find that synthetic shelterin bridge lacking cooperativity requires a linker pair that matches the native bridge in complex lifespan but has dramatically higher affinity. We find that cooperative assembly confers kinetic properties on the shelterin bridge allowing disassembly to function as a molecular timer, regulating the duration of the telomere open state, and consequently telomere lengthening to achieve a defined species-specific length range.

Chromosomes; Telomere; Humans; Schizosaccharomyces; Multiprotein Complexes; DNA-Binding Proteins; Telomere-Binding Proteins; Schizosaccharomyces pombe Proteins; DNA; Single-Stranded; Kinetics; Mutation; Telomere Homeostasis; 1.1 Normal biological development and functioning; Generic health relevance; Developmental Biology; Environmental Sciences; Biological Sciences; Information and Computing Sciences; article

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Nucleic acids research vol 49, iss 14, 8110-8119 0305-1048

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