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Metabonomics and the gut microbiome associated with primary response to Anti-TNF therapy in Crohn’s Disease
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- المؤلفون: Ding, N.S.; McDonald, J.A.K.; Perdones-Montero, A.; Rees, D.N.; Adegbola, S.O.; Misra, R.; Hendy, P.; Penez, L.; Marchesi, J.R.; Holmes, E.; Sarafian, M.H.; Hart, A.L.
- المصدر:
Ding, N.S., McDonald, J.A.K., Perdones-Montero, A., Rees, D.N., Adegbola, S.O., Misra, R., Hendy, P., Penez, L., Marchesi, J.R., Holmes, E. <
- نوع التسجيلة:
Electronic Resource
- الدخول الالكتروني :
https://researchrepository.murdoch.edu.au/id/eprint/57717/
https://researchrepository.murdoch.edu.au/id/eprint/57717
full_text_status:none
- معلومة اضافية
- Publisher Information:
Elsevier B.V. 2020
- نبذة مختصرة :
Background and Aims Anti-tumour necrosis factor [anti-TNF] therapy is indicated for treatment of moderate to severe inflammatory bowel disease [IBD], but has a primary non-response rate of around 30%. We aim to use metabonomic and metataxonomic profiling to identify predictive biomarkers of anti-TNF response in Crohn’s disease. Methods Patients with luminal Crohn’s disease, commencing anti-TNF therapy, were recruited with urine, faeces, and serum samples being collected at baseline and 3-monthly. Primary response was defined according to a combination of clinical and objective markers of inflammation. Samples were measured using three UPLC-MS assays: lipid, bile acid, and Hydrophillic Interaction Liquid Chromatography [HILIC] profiling with 16S rRNA gene sequencing of faeces. Results Samples were collected from 76 Crohn’s disease patients who were anti-TNF naïve and from 13 healthy controls. There were 11 responders, 37 non-responders, and 28 partial responders in anti-TNF-treated Crohn’s patients. Histidine and cysteine were identified as biomarkers of response from polar metabolite profiling [HILIC] of serum and urine. Lipid profiling of serum and faeces found phosphocholines, ceramides, sphingomyelins, and triglycerides, and bile acid profiling identified primary bile acids to be associated with non-response to anti-TNF therapy, with higher levels of phase 2 conjugates in non-responders. Receiver operating curves for treatment response demonstrated 0.94 +/ -0.10 [faecal lipid], 0.81 +/- 0.17 [faecal bile acid], and 0.74 +/- 0.15 [serum bile acid] predictive ability for anti-TNF response in Crohn’s disease. Conclusions This prospective, longitudinal cohort study of metabonomic and 16S rRNA gene sequencing analysis demonstrates that a range of metabolic biomarkers involving lipid, bile acid, and amino acid pathways may contribute to prediction of response to anti-TNF therapy in Crohn’s disease.
- الموضوع:
- Note:
English
- Other Numbers:
LD1 oai:researchrepository.murdoch.edu.au:57717
https://researchrepository.murdoch.edu.au/id/eprint/57717/
1276815535
- Contributing Source:
MURDOCH UNIV LIBR
From OAIster®, provided by the OCLC Cooperative.
- الرقم المعرف:
edsoai.on1276815535
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