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Molecular cloning and expression analysis of Ganoderma boninense cyclophilins at different growth and infection stages

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اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • Publisher Information:
      Academic Press 2017
    • نبذة مختصرة :
      Oil palm is subjected to various diseases including Basal Stem Rot (BSR) which is mainly caused by the basidiomycetes fungi, Ganoderma. However, studies on the fungal infection mechanism especially at molecular level and the biological processes involved are still very limited. Fungal cyclophilin (CYP) has been reported to be involved in fungal pathogenicity but involvement of CYP in the pathogenicity of Ganoderma boninense has not been revealed yet. The main objective of this study is to isolate cDNAs encoding CYP and to profile the expression level of these transcripts during infection stages of G. boninense. In this study, five full-length cDNAs encoding CYP were successfully isolated from G. boninense. They were classified as different family members of CYP because significant differences could be observed on their coding, 5′ or 3′ un-translated regions (UTRs). The expression of these five CYP transcripts in different type of G. boninense tissues and the infecting mycelia tissues was studied using real time quantitative PCR (qPCR). Based on the expression results and analysis, the potential functions of these CYP transcripts were predicted to be involved in the development of fruiting body (GbCYP201) and fungal stress response or pathogenicity (GbCYP203 and GbCYP205).
    • الموضوع:
      Article; PeerReviewed
    • Availability:
      Open access content. Open access content
    • Note:
      text
      English
    • Other Numbers:
      MYPUT oai:psasir.upm.edu.my:62468
      Lim, Fook Hwa and Iskandar, Nor Fakhrana and Abd Rasid, Omar and Idris, Abu Seman and Ho, Chai Ling and Shaharuddin, Noor Azmi and Ahmad Parveez, Ghulam Kadir (2017) Molecular cloning and expression analysis of Ganoderma boninense cyclophilins at different growth and infection stages. Physiological and Molecular Plant Pathology, 99. 31 - 40. ISSN 0885-5765; ESSN: 1096-1178
      1252974605
    • Contributing Source:
      UNIVERSITI PUTRA MALAYSIA
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1252974605
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