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Nano-liquid Chromatography-orbitrap MS-based Quantitative Proteomics Reveals Differences Between the Mechanisms of Action of Carnosic Acid and Carnosol in Colon Cancer Cells

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  • معلومة اضافية
    • Publisher Information:
      Uppsala universitet, Analytisk kemi Uppsala universitet, Science for Life Laboratory, SciLifeLab CSIC, CIAL, Food Sci Res Inst, Lab Food, Calle Nicolas Cabrera 9, Madrid 28049, Spain. CSIC, CIAL, Food Sci Res Inst, Lab Food, Calle Nicolas Cabrera 9, Madrid 28049, Spain. CSIC, CIAL, Food Sci Res Inst, Lab Food, Calle Nicolas Cabrera 9, Madrid 28049, Spain. CSIC, CIAL, Food Sci Res Inst, Lab Food, Calle Nicolas Cabrera 9, Madrid 28049, Spain. 2017
    • نبذة مختصرة :
      Carnosic acid (CA) and carnosol (CS) are two structurally related diterpenes present in rosemary herb (Rosmarinus officinalis). Although several studies have demonstrated that both diterpenes can scavenge free radicals and interfere in cellular processes such as cell proliferation, they may not necessarily exert the same effects at the molecular level. In this work, a shotgun proteomics study based on stable isotope dimethyl labeling (DML) and nano -liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) has been performed to identify the relative changes in proteins and to gain some light on the specific molecular targets and mechanisms of action of CA and CS in HT-29 colon cancer cells. Protein profiles revealed that CA and CS induce different Nrf2-mediated response. Furthermore, examination of our data revealed that each diterpene affects protein homeostasis by different mechanisms. CA treatment induces the expression of proteins involved in the unfolded protein response in a concentration dependent manner reflecting ER stress, whereas CS directly inhibits chymotrypsin-like activity of the 20S proteasome. In conclusion, the unbiased proteomics-wide method applied in the present study has demonstrated to be a powerful tool to reveal differences on the mechanisms of action of two related bioactive compounds in the same biological model.
    • الموضوع:
      Biochemistry and Molecular Biology; Biokemi och molekylärbiologi; Article in journal; info:eu-repo/semantics/article; text
    • الرقم المعرف:
      10.1074.mcp.M116.061481
    • Availability:
      Open access content. Open access content
      info:eu-repo/semantics/restrictedAccess
    • Note:
      English
    • Other Numbers:
      UPE oai:DiVA.org:uu-316126
      doi:10.1074/mcp.M116.061481
      PMID 27834734
      ISI:000392205300002
      1235304154
    • Contributing Source:
      UPPSALA UNIV LIBR
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1235304154
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