Random monoallelic expression of the imprinted IGF2 and H19 genes in the absence of discriminative parental marks

Uppsala universitet, Institutionen för fysiologi och utvecklingsbiologi Uppsala universitet, Zoologisk utvecklingsbiologi Institutionen för kvinnors och barns hälsa Uppsala universitet, Zoologisk utvecklingsbiologi zoologisk utvecklingsbiologi 1999

PDGFB is a growth factor which is vital for the completion of normal prenatal development. In this study, we report the phenotypic analysis of placentas from mouse conceptuses that lack a functional PDGFB or PDGFRbeta gene. Placentas of both types of mutant exhibit changes in the labyrinthine layer, including dilated embryonic blood vessels and reduced numbers of both pericytes and trophoblasts. These changes are seen from embryonic day (E) 13.5, which coincides with the upregulation of PDGFB mRNA levels in normal placentas. By E17, modifications in shape, size, and number of the fetal blood vessels in the mutant placentas cause an abnormal ratio of the surface areas between the fetal and the maternal blood vessels in the labyrinthine layer. Our data suggest that PDGFB acts locally to contribute to the development of the labyrinthine layer of the fetal placenta and the formation of a proper nutrient-waste exchange system during fetal development. We point out that the roles of PDGFB/Rbeta signaling in the placenta may be analogous to those in the developing kidney, by controlling pericytes in the labyrinthine layer and mesangial cells in the kidney.

Animals; Capillaries/anatomy & histology; Female; Kidney/embryology; Maternal-Fetal Exchange; Mice; Mice; Knockout; Models; Biological; Pericytes/metabolism; Placenta/*anatomy & histology/blood supply/ultrastructure; Platelet-Derived Growth Factor/analysis/*physiology; Pregnancy; Proto-Oncogene Proteins/analysis/*physiology; Proto-Oncogene Proteins c-sis; Receptors; Platelet-Derived Growth Factor/analysis/blood/physiology; Research Support; Non-U.S. Gov't; Research Support; U.S. Gov't; P.H.S.; Time Factors; Trophoblasts/metabolism; Article in journal; info:eu-repo/semantics/article; text

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