EHD2 regulates adipocyte function and is enriched at cell surface-associated lipid droplets in primary human adipocytes

Umeå universitet, Institutionen för medicinsk kemi och biofysik AMER SOC CELL BIOLOGY 2019 info:eu-repo/date/embargoEnd/2019-07-01

Adipocytes play a central role in energy balance, and dysfunctional adipose tissue severely affects systemic energy homeostasis. The ATPase EH domain-containing 2 (EHD2) has previously been shown to regulate caveolae, plasma membrane-specific domains that are involved in lipid uptake and signal transduction. Here, we investigated the role of EHD2 in adipocyte function. We demonstrate that EHD2 protein expression is highly up-regulated at the onset of triglyceride accumulation during adipocyte differentiation. Small interfering RNA-mediated EHD2 silencing affected the differentiation process and impaired insulin sensitivity, lipid storage capacity, and lipolysis. Fluorescence imaging revealed localization of EHD2 to caveolae, close to cell surface-associated lipid droplets in primary human adipocytes. These lipid droplets stained positive for glycerol transporter aquaporin 7 and phosphorylated perilipin-1 following adrenergic stimulation. Further, EHD2 overexpression in human adipocytes increased the lipolytic signaling and suppressed the activity of transcription factor PPAR.. Overall, these data suggest that EHD2 plays a key role for adipocyte function.

Cell Biology; Cellbiologi; Article in journal; info:eu-repo/semantics/article; text

10.1091.mbc.E18-10-0680

Open access content. Open access content
info:eu-repo/semantics/embargoedAccess

application/pdf
English

UPE oai:DiVA.org:umu-158942
doi:10.1091/mbc.E18-10-0680
PMID 30811273
ISI:000466151300003
1234609873

UPPSALA UNIV LIBR
From OAIster®, provided by the OCLC Cooperative.

edsoai.on1234609873