IL-7 mediates Ebf-1-dependent lineage restriction in early lymphoid progenitors

Experimentell hematologi Hälsouniversitetet Lund University American Society of Hematology 2011

eficiencies in the IL-7 signaling pathway result in severe disruptions of lymphoid development in adult mice. To understand more about how IL-7 deficiency impacts early lymphoid development, we have investigated lineage restriction events within the common lymphoid progenitor (CLP) compartment in IL-7 knockout mice. This revealed that although IL-7 deficiency had a minor impact on the development of LY6D(-) multipotent CLPs, the formation of the lineage restricted LY6D(+) CLP population was dramatically reduced. This was reflected in a low-level transcription of B-lineage genes as well as in a loss of functional B-cell commitment. The few Ly6D(+) CLPs developed in the absence of IL-7 displayed increased lineage plasticity and low expression of Ebf-1. Absence of Ebf-1 could be linked to increased plasticity because even though Ly6D(+) cells develop in Ebf-1-deficient mice, these cells retain both natural killer and dendritic cell potential. This reveals that IL-7 is essential for normal development of Ly6D(+) CLPs and that Ebf-1 is crucial for lineage restriction in early lymphoid progenitors.

Medical and Health Sciences; Medicin och hälsovetenskap; Article in journal; info:eu-repo/semantics/article; text

10.1182.blood-2011-01-332189

English

UPE oai:DiVA.org:liu-70104
doi:10.1182/blood-2011-01-332189
ISI:000293510000020
1234239776

UPPSALA UNIV LIBR
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