Estrogen-dependent signaling in a molecularly distinct subclass of aggressive prostate cancer

Örebro universitet, Hälsoakademin Oxford 2008

BACKGROUND: The majority of prostate cancers harbor gene fusions of the 5'-untranslated region of the androgen-regulated transmembrane protease serine 2 (TMPRSS2) promoter with erythroblast transformation-specific transcription factor family members. The common fusion between TMPRESS2 and v-ets erythroblastosis virus E26 oncogene homolog (avian) (ERG) is associated with a more aggressive clinical phenotype, implying the existence of a distinct subclass of prostate cancer defined by this fusion. METHODS: We used complementary DNA-mediated annealing, selection, ligation, and extension to determine the expression profiles of 6144 transcriptionally informative genes in archived biopsy samples from 455 prostate cancer patients in the Swedish Watchful Waiting cohort (1987-1999) and the United States-based Physicians(') Health Study cohort (1983-2003). A gene expression signature for prostate cancers with the TMPRSS2-ERG fusion was determined using partitioning and classification models and used in computational functional analysis. Cell proliferation and TMPRSS2-ERG expression in androgen receptor-negative (NCI-H660) prostate cancer cells after treatment with vehicle or estrogenic compounds were assessed by viability assays and quantitative polymerase chain reaction, respectively. All statistical tests were two-sided. RESULTS: We identified an 87-gene expression signature that distinguishes TMPRSS2-ERG fusion prostate cancer as a discrete molecular entity (area under the curve = 0.80, 95% confidence interval [CI] = 0.792 to 0.81; P < .001). Computational analysis suggested that this fusion signature was associated with estrogen receptor (ER) signaling. Viability of NCI-H660 cells decreased after treatment with estrogen (viability normalized to day 0, estrogen vs vehicle at day 8, mean = 2.04 vs 3.40, difference = 1.36, 95% CI = 1.12 to 1.62) or ERbeta agonist (ERbeta agonist vs vehicle at day 8, mean = 1.86 vs 3.40, difference = 1.54, 95% CI = 1.39 to 1.69) but increas

Antineoplastic Agents; Hormonal/*pharmacology/therapeutic use; Area Under Curve; Blotting; Western; Cell Line; Tumor; Cell Survival/drug effects; Chromatin Immunoprecipitation; DNA; Complementary/metabolism; Estradiol/pharmacology; Estrogen Receptor alpha/*antagonists & inhibitors/metabolism; Estrogen Receptor beta/*agonists/metabolism; Estrogens/*metabolism; Gene Expression Profiling; Gene Expression Regulation; Neoplastic/drug effects; Health Surveys; Humans; Male; Neoplasms; Hormone-Dependent/drug therapy/*metabolism/pathology; Nitriles/pharmacology; Oncogene Proteins; Fusion/*metabolism; Physicians/statistics & numerical data; Polymerase Chain Reaction; Propionates/pharmacology; Prostatic Neoplasms/drug therapy/*metabolism/pathology; Pyrazoles/pharmacology; Serine Endopeptidases/metabolism; Signal Transduction/drug effects; Sweden/epidemiology; Transfection; Medical and Health Sciences; Medicin och hälsovetenskap; Cancer and Oncology; Cancer och onkologi; Urology and Nephrology; Urologi och njurmedicin; Article in journal; info:eu-repo/semantics/article; text

10.1093.jnci.djn150

Open access content. Open access content
info:eu-repo/semantics/restrictedAccess

English

UPE oai:DiVA.org:oru-3670
0000-0002-3649-2639
doi:10.1093/jnci/djn150
PMID 18505969
ISI:000256522900012
Scopus 2-s2.0-44949204525
1233784729

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