Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal

CELL PRESS 2019

Generation of neuronal types at the right time, location, and number is essential for building a functional nervous system. Significant progress has been reached in understanding the mechanisms that govern neuronal diversity. Cerebrospinal fluid-contacting neurons (CSF-cNs), an intriguing spinal cord central canal population, are produced during advanced developmental stages, simultaneous with glial and ependymal cells. It is unknown how CSF-cNs are specified after the neurogenesis-to-gliogenesis switch. Here, we identify delayed Ascl1 expression in mouse spinal progenitors during the gliogenic phase as key in CSF-cN differentiation. With fate mappings and time-controlled deletions, we demonstrate that CSF-cNs derive from Ascl1-expressing cells and that Ascl1 triggers late neurogenesis in the amniote spinal cord. Ascl1 abrogation transforms prospective CSF-cN progenitors into ependymocytes. These results demonstrate that late spinal progenitors have the potential to produce neurons and that Ascl1 initiates CSF-cN differentiation, controlling the precise neuronal and nonneuronal composition of the spinal central canal.

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English

K7U oai:USBKOELN.ub.uni-koeln.de:14388
Di Bella, Daniela J., Carcagno, Abel L., Lucia Bartolomeu, M., Belen Pardi, M., Loehr, Heiko, Siegel, Nicole, Hammerschmidt, Matthias, Marin-Burgin, Antonia and Lanuza, Guillermo M. (2019). Ascl1 Balances Neuronal versus Ependymal Fate in the Spinal Cord Central Canal. Cell Reports, 28 (9). S. 2264 - 2278. CAMBRIDGE: CELL PRESS. ISSN 2211-1247
1201315233

UNIVERSITATS- UND STADTBIBLIOTHEK KOLN
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