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From clinicogenetic studies of maturity-onset diabetes of the young to unraveling complex mechanisms of glucokinase regulation.

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  • معلومة اضافية
    • Publisher Information:
      2006
    • نبذة مختصرة :
      Glucokinase functions as a glucose sensor in pancreatic beta-cells and regulates hepatic glucose metabolism. A total of 83 probands were referred for a diagnostic screening of mutations in the glucokinase (GCK) gene. We found 11 different mutations (V62A, G72R, L146R, A208T, M210K, Y215X, S263P, E339G, R377C, S453L, and IVS5 + 1G>C) in 14 probands. Functional characterization of recombinant glutathionyl S-transferase-G72R glucokinase showed slightly increased activity, whereas S263P and G264S had near-normal activity. The other point mutations were inactivating. S263P showed marked thermal instability, whereas the stability of G72R and G264S differed only slightly from that of wild type. G72R and M210K did not respond to an allosteric glucokinase activator (GKA) or the hepatic glucokinase regulatory protein (GKRP). Mutation analysis of the role of glycine at position 72 by substituting E, F, K, M, S, or Q showed that G is unique since all these mutants had very low or no activity and were refractory to GKRP and GKA. Structural analysis provided plausible explanations for the drug resistance of G72R and M210K. Our study provides further evidence that protein instability in combination with loss of control by a putative endogenous activator and GKRP could be involved in the development of hyperglycemia in maturity-onset diabetes of the young, type 2. Furthermore, based on data obtained on G264S, we propose that other and still unknown mechanisms participate in the regulation of glucokinase.
    • الموضوع:
      Binding Sites; Blood Glucose/metabolism; Carrier Proteins/metabolism; Crystallography, X-Ray; Diabetes Mellitus, Type 2/genetics/pathology/prevention & control; Enzyme Stability/drug effects; Genetic Testing; Glucokinase/chemistry/genetics/metabolism; Glucose/pharmacology; Humans; Hyperglycemia/enzymology/genetics/metabolism; Kinetics; Mutant Proteins/chemistry/metabolism; Mutation; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; info:eu-repo/semantics/article
    • الرقم المعرف:
      10.2337.db05-1513
    • Availability:
      Open access content. Open access content
    • Note:
      English
    • Other Numbers:
      LULUX oai:orbilu.uni.lu:10993/27204
      DOI:10.2337/db05-1513
      WOS:000238053400020
      PMID:16731834
      SCOPUS_ID:33748302011
      1135479655
    • Contributing Source:
      UNIV OF LUXEMBOURG
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1135479655
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