Sequence analysis of polymerase chain reaction amplified t(14;18) chromosomal breakpoints in formalin fixed, paraffin wax embedded follicular lymphoma.

1992

AIMS: To determine whether junctional sequences of rearranged chromosomes can be amplified by use of the polymerase chain reaction (PCR) and whether direct sequence analysis of the PCR products is possible, using DNA from formalin fixed, paraffin wax embedded biopsy specimens. METHODS: DNA was extracted from paraffin wax embedded, formalin fixed lymphoma specimens, and junctional sequences of rearranged chromosomes were amplified by the PCR. The products were used as templates for asymmetrical PCR. Subsequently, direct sequence analysis was performed using the chain termination method. RESULTS: Formalin fixed, paraffin wax embedded biopsy specimens and PCR amplification could be used to determine the nucleotide sequences of junctional regions of rearranged chromosomes t(14;18) from patients with follicular lymphoma. CONCLUSION: The identification of junctional sequences of the translocation in follicular lymphoma provides a molecular "fingerprint" of t(14;18) of the lymphoma of an individual patient and can be used for the detection of clone specific DNA in any biopsy tissue obtained from the patient. The strategy used for rapid sequence analysis of PCR amplified DNA sequences will be useful in many areas of molecular pathology.

Base Sequence; Chromosomes, Human, Pair 14; Chromosomes, Human, Pair 18; DNA, Neoplasm/chemistry; Formaldehyde; Humans; Lymphoma, Follicular/genetics; Molecular Sequence Data; Paraffin Embedding; Polymerase Chain Reaction; Translocation, Genetic/genetics; info:eu-repo/semantics/article

Open access content. Open access content
info:eu-repo/semantics/restrictedAccess

English

LULUX oai:orbilu.uni.lu:10993/18344
info:doi:10.1136/jcp.45.3.210
PMID:1556227
SCOPUS_ID:0026575303
WOS:A1992HH45500006
1135478996

UNIV OF LUXEMBOURG
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