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A coding RNA sequence acts as a replication signal in cardioviruses.

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اقرأ أكثر حفظ في قائمتي
  • المصدر:
    Proceedings of the National academy of sciences of the United States of America, Vol. 96, no. 20, p. 11560-11565 (1999)
  • نوع التسجيلة:
    Electronic Resource
  • الدخول الالكتروني :
    http://hdl.handle.net/2078.1/8359
  • معلومة اضافية
    • Publisher Information:
      National academy of sciences 1999
    • Added Details:
      UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique
      Lobert, P E
      Escriou, N
      Ruelle, Jean
      Michiels, Thomas
    • نبذة مختصرة :
      Theiler's virus and Mengo virus are representatives of the Cardiovirus genus within the picornavirus family. Their genome is an 8-kilobase long positive strand RNA molecule. This RNA molecule plays three roles in infected cells: It serves as a messenger RNA, acts as a template for genome replication, and is encapsidated to form progeny virions. We observed that a cis-acting signal required for replication of Theiler's virus was contained within a 130-nt stretch of the region encoding the capsid protein VP2. This RNA sequence does not influence internal ribosome entry site-mediated translation initiation and thus likely acts directly as a signal for the replication complex. We found a similar signal in the VP2-coding sequence of Mengo virus, and both signals could be functionally exchanged. Within the replication element, a 9-nt sequence that is highly conserved among cardioviruses was shown to be essential for replication. This conserved sequence was contained in mostly unpaired regions of the RNA secondary structure predicted for the replication elements of the various cardioviruses. Interestingly, a similar replication element has been reported to occur in the distantly related human rhinovirus type 14, suggesting that such elements could be conserved throughout the picornavirus family. However, the different location of the replication elements in rhinovirus and cardioviruses, and the fact that they were not functionally exchangeable, is raising intriguing questions about the evolution of such signals in picornaviruses.
    • الموضوع:
      Capsid; Capsid Proteins; Cardiovirus; Cells, Cultured; Humans; Protein Biosynthesis; RNA, Viral; Rhinovirus; Theilovirus; Virus Replication; info:eu-repo/semantics/article
    • Note:
      English
    • Other Numbers:
      UCDLC oai:dial.uclouvain.be:boreal:8359
      boreal:8359
      info:doi/10.1073/pnas.96.20.11560
      info:pmid/10500216
      urn:ISSN:0027-8424
      urn:EISSN:1091-6490
      1130586433
    • Contributing Source:
      UNIVERSITE CATHOLIQUE DE LOUVAIN
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.on1130586433
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