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IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts

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  • نوع التسجيلة:
    Electronic Resource
  • الدخول الالكتروني :
    https://www.dovepress.com/igg-from-patients-with-systemic-sclerosis-bind-to-dna-antitopoisomeras-peer-reviewed-fulltext-article-BTT
    info:eu-repo/semantics/altIdentifier/doi/10.2147/BTT.S3188
  • معلومة اضافية
    • Publisher Information:
      Dove Press 2008-09-12
    • Added Details:
      Tamby,Mathieu C
      Servettaz,Amélie
      Tamas,Nicolas
      Reinbolt,Joseph
      Caux,Frédéric
      Meyer,Olivier
      Allanore,Yannick
      Kahan,André
      Guillevin,Loïc
      Mouthon,Luc
    • نبذة مختصرة :
      Mathieu C Tamby1, Amélie Servettaz1, Nicolas Tamas1, Joseph Reinbolt2, Frédéric Caux3, Olivier Meyer4, Yannick Allanore5, André Kahan5, Loïc Guillevin6, Luc Mouthon1,61Paris-Descartes University, Faculty of Medicine, UPRES-EA 4058, Paris, France; 2UPR 9002, Centre National de la Recherche Scientifique, Strasbourg, France; 3UPRES EA 2436, Paris-Nord University, Bobigny, France; 4Rheumatology Department, Bichat Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France; 5Rheumatology A Department, Cochin Hospital, AP-HP; 6Paris-Descartes University, Faculty of Medicine, Department of Internal Medicine and French Reference Center for Necrotizing Vasculitides and Systemic Sclerosis, Cochin Hospital, AP-HP, Paris, FranceAbstract: By using a semi-quantitative immunoblotting technique, we have analyzed serum immunoglobulin G (IgG) reactivities of patients with limited cutaneous systemic sclerosis and anticentromere antibodies, patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies, patients with diffuse systemic sclerosis without antitopoisomerase 1 or anticentromere antibodies and age- and gender-matched healthy controls with normal human skin fibroblasts and HEp-2 cells antigens. Serum IgG reactivities of patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies differed significantly from those of healthy controls or systemic sclerosis patients in other groups for reactivity with fibroblast proteins. IgG from patients with antitopoisomerase 1 antibodies bound to a 90 kDa fibroblast band and to a 100 kDa protein band in a HEp-2 cell protein extract. These two bands were further identified as DNA topoisomerase 1. Our results indicate that IgG from patients with diffuse systemic sclerosis bind DNA topoisomerase 1 in normal human fibroblasts extracts.Keywords: systemic sclerosis, autoantibodies, IgG, fibroblast, DNA topoisomerase 1
    • الموضوع:
      Biologics: Targets and Therapy; Original Research; info:eu-repo/semantics/article
    • Note:
      text/html
      English
    • Other Numbers:
      NZDMP oai:dovepress.com/2308
      953554903
    • Contributing Source:
      DOVE MEDL PRESS LTD
      From OAIster®, provided by the OCLC Cooperative.
    • الرقم المعرف:
      edsoai.ocn953554903
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