Heterogeneity and evolution of thymidine kinase and DNA polymerase mutants of herpes simplex virus type 1: Implications for antiviral therapy

2013-04

Background. Infections caused by acyclovir-resistant isolates of herpes simplex virus (HSV) after hematopoietic stem cell transplantation (HSCT) are an emerging concern. An understanding of the evolutionary aspects of HSV infection is crucial to the design of effective therapeutic and control strategies.Methods. Eight sequential HSV-1 isolates were recovered from an HSCT patient who suffered from recurrent herpetic gingivostomatitis and was treated alternatively with acyclovir, ganciclovir, and foscavir. The diverse spectra and temporal changes of HSV drug resistance were determined phenotypically (drug-resistance profiling) and genotypically (sequencing of the viral thymidine kinase and DNA polymerase genes).Results. Analysis of 60 clones recovered from the different isolates demonstrated that most of these isolates were heterogeneous mixtures of variants, indicating the simultaneous infection with different drug-resistant viruses. The phenotype/genotype of several clones associated with resistance to acyclovir and/or foscavir were identified. Two novel mutations (E798K and I922T) in the viral DNA polymerase could be linked to drug resistance.Conclusions. The heterogeneity within the viral populations and the temporal changes of drug-resistant viruses found in this HSCT recipient were remarkable, showing a rapid evolution of HSV-1. Drug-resistance surveillance is highly recommended among immunocompromised patients to manage the clinical syndrome and to avoid the emergence of multidrug-resistant isolates. © 2013 The Author.
SCOPUS: ar.j
info:eu-repo/semantics/published

Allergie et immunopathologie; Pathologie maladies infectieuses; Acyclovir -- pharmacology; Adult; DNA, Viral -- analysis -- genetics; Drug Resistance, Multiple, Viral; Evolution, Molecular; Female; Ganciclovir -- pharmacology; Genes, pol; Genetic Heterogeneity; Genotype; Hematopoietic Stem Cell Transplantation; Herpesvirus 1, Human -- enzymology -- genetics -- isolation & purification; Humans; Leukemia, Myeloid, Acute -- pathology; Mutation; Phenotype; Stomatitis, Herpetic -- drug therapy -- pathology -- virology; Thymidine Kinase -- genetics; DNA polymerase; drug-resistance dynamics in HSV; evolution of HSV populations; hematopoietic stem cell transplantation (HSCT); heterogenicity of HSV populations; multiple HSV infections; thymidine kinase; info:eu-repo/semantics/article; info:ulb-repo/semantics/articlePeerReview; info:ulb-repo/semantics/openurl/article

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English

EQY oai:dipot.ulb.ac.be:2013/168431
uri/info:doi/10.1093/infdis/jit019
uri/info:pii/jit019
uri/info:pmid/23315323
uri/info:scp/84875605435
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