HRP-based biosensor for monitoring rifampicin

2003

Pyrrole was electropolymerized onto a Pt electrode in the presence of LiClO(4) and horseradish peroxidase (HRP). This HRP-based biosensor has been used for the amperometric detection of rifampicin (RIF) in the presence of a constant concentration of H(2)O(2). The C(H(2)O(2)) as well as the applied potential (E(ap)) and the pH of the phosphate buffer have simultaneously been optimized through a central composite design. Under these conditions, repeatability, reproducibility, and stability of the modified electrode have been analyzed. The detection limit for RIF has been calculated taking into account the probability of false-positive (alpha) and -negative (beta), reaching a value of 5.06x10(-6) mol dm(-3). The biosensor was applied to the determination of RIF in pharmaceutical preparations and biological samples.
Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't
Validation Studies
info:eu-repo/semantics/published

Chimie analytique; Biosensing Techniques -- instrumentation; Biosensing Techniques -- methods; Coated Materials, Biocompatible -- chemistry; Drug Contamination -- prevention & control; Electrochemistry -- instrumentation; Electrochemistry -- methods; Enzymes, Immobilized -- chemistry; Horseradish Peroxidase -- chemistry; Polymers -- chemistry; Pyrroles -- chemistry; Reproducibility of Results; Rifampin -- analysis; Rifampin -- chemistry; Sensitivity and Specificity; Urinalysis -- instrumentation; Urinalysis -- methods; Electrodeposited polypyrrole; Experimental design; HRP biosensor; Rifampicin; info:eu-repo/semantics/article; info:ulb-repo/semantics/articlePeerReview; info:ulb-repo/semantics/openurl/article

Open access content. Open access content
1 full-text file(s): info:eu-repo/semantics/restrictedAccess

1 full-text file(s): application/pdf
English

EQY oai:dipot.ulb.ac.be:2013/26843
uri/info:doi/10.1016/S0956-5663(02)00251-8
uri/info:pii/S0956566302002518
uri/info:pmid/12788559
uri/info:scp/0038022664
local/jmk-0123
778634143

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