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Influences of Molecular Weights on Physicochemical and Biological Properties of Collagen-Alginate Scaffolds

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  • معلومة اضافية
    • بيانات النشر:
      MDPI AG, 2021.
    • الموضوع:
      2021
    • Collection:
      LCC:Biology (General)
    • نبذة مختصرة :
      For tissue engineering applications, biodegradable scaffolds containing high molecular weights (MW) of collagen and sodium alginate have been developed and characterized. However, the properties of low MW collagen-based scaffolds have not been studied in previous research. This work examined the distinctive properties of low MW collagen-based scaffolds with alginate unmodified and modified by subcritical water. Besides, we developed a facile method to cross-link water-soluble scaffolds using glutaraldehyde in an aqueous ethanol solution. The prepared cross-linked scaffolds showed good structural properties with high porosity (~93%) and high cross-linking degree (50–60%). Compared with collagen (6000 Da)-based scaffolds, collagen (25,000 Da)-based scaffolds exhibited higher stability against collagenase degradation and lower weight loss in phosphate buffer pH 7.4. Collagen (25,000 Da)-based scaffolds with modified alginate tended to improve antioxidant capacity compared with scaffolds containing unmodified alginate. Interestingly, in vitro coagulant activity assay demonstrated that collagen (25,000 Da)-based scaffolds with modified alginate (C25-A63 and C25-A21) significantly reduced the clotting time of human plasma compared with scaffolds consisting of unmodified alginate. Although some further investigations need to be done, collagen (25,000 Da)-based scaffolds with modified alginate should be considered as a potential candidate for tissue engineering applications.
    • File Description:
      electronic resource
    • ISSN:
      1660-3397
    • Relation:
      https://www.mdpi.com/1660-3397/19/2/85; https://doaj.org/toc/1660-3397
    • الرقم المعرف:
      10.3390/md19020085
    • الرقم المعرف:
      edsdoj.f780adb7b6c848eabf082514838a29af