نبذة مختصرة : Background: /purpose: The quality of the hybrid layer is of great importance for dentin bonding. The purpose of this study was to develop a novel copper-based pretreatment and investigate the effect of the pretreatment combined with universal adhesives on the dentin bond strength. Materials and methods: Etch-and-rinse adhesive Single Bond 2 (SB2) and two universal adhesives Prime Bond Universal (PBU) and Single Bond Universal (SBU) were selected. The dentin surfaces were pretreated with CuSO4 solution and K2HPO4 solution in turn (Cu–P pretreatment), and the adhesive was applied following the manufacturer's instructions. There were four groups of Cu–P pretreatment: HH-Cu (1.5 mol/L CuSO4 + 1.0 mol/L K2HPO4); H–Cu (0.15 mol/L CuSO4 + 0.1 mol/L K2HPO4); L-Cu (0.015 mol/L CuSO4 + 0.01 mol/L K2HPO4); and LL-Cu (0.0015 mol/L CuSO4 + 0.001 mol/L K2HPO4). The microtensile bond strength (μ-TBS) and fracture mode were determined. The dentin surface after pretreatment and the antimicrobial properties of the pretreatment agent were also evaluated. Results: The minimum inhibitory concentration and minimum bactericidal concentration of Cu–P pretreatment were 0.012 mol/L CuSO4 + 0.008 mol/L K2HPO4. Combined with SB2, the H–Cu and L-Cu groups showed a higher μ-TBS (P
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