DETECTION OF SPECIFIC ANTIBODIES TO HSV-1 WITH THE OPTICAL BIOSENSOR

Aim. To investigate the possibility of using the optoelectronic biosensor «Plasmon-6» based on surface plasmon resonance (SPR) for detection of specific antibodies to herpes simplex virus type 1 (HSV-1). Methods. HSV-1 was propagated in MDBK (Madin-Darby Bovine Kidney) cell culture and was purified by differential centrifugation in cesium chloride density gradient. The presence of viral proteins was tested by polyacrylamide gel electrophoresis. Viral proteins were applied on the biochip surface pre-modified by dextran. Selection of human blood sera was performed by ELISA using the test systems «HSV-1 IgG ELISA» (GenWay, USA) and «HSV-1 IgG ELISA» (Vector-Best, Russia). Biosensor analysis was carried out on the optoelectronic spectrometer «Plasmon-6». Results. Concentrated preparation of HSV-1 proteins was obtained. It was shown that application of viral proteins at 8x10-5 mg / mm2 was optimal for detection of antibodies against HSV-1. 10 negative to HSV-1 blood sera of donors have been tested on biochips to determine the limits of positive and negative response. The comparative analysis of 25 blood sera of the patients for detection the antibodies to HSV-1 was carried out by ELISA and SPR methods. The coincidence of the results of SPR-analysis and ELISA was in 24 of 25 cases. Conclusion. As a result of carried out investigations there were shown that immunosensor analysis using the device «Plasmon-6» as well as traditional approaches allowed to detect specific antibodies to HSV-1 in blood sera.