Storage of Actinobacteria of the Genera Streptomyces and Nonomuraea by Low Temperature Preservation

The influence of storage of actinobacteria Streptomyces hygroscopicus RIA 1433T, Nonomuraea roseoviolacea subsp. carminata INA 4281 and Nonomuraea sp. INA 34-06 at extremely low temperatures (-70°С) for 1.5 years was studied with respect to their viability and antibiotic activity. The spores of the actinobacteria preserved their high viability when freezed at a concentration of 105-107 CFU/ml. As for the antibiotic activity against the test culture Micrococcus luteus ATCC 9341, the strains differed: the S.hygroscopicus RIA 1433T colonies preserved their antibiotic activity against the test culture, the antibiotic activity of Nonomuraea roseoviolacea subsp. carminata lowered by 5% and that of N. sp. INA 34-06 lowered by 44%. Differences in the resistance of the strains to the storage at the extremely low temperatures were observed when the suspensions contained low concentrations of the spores (102 CFU/ml): S.hygroscopicus RIA 1433T preserved its viability and antibiotic activity during 1.5 years, while N.roseoviolacea subsp. carminata INA 4281 and N. sp. INA 34-06 lost the viability by the 8th month of the storage. The study showed that 10% glycerol solution used as a cryoprotector during the storage had no effect on viability and antibiotic activity of the actinobacteria.