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Comparative and integrative single cell analysis reveals new insights into the transcriptional immaturity of stem cell-derived β cells

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  • معلومة اضافية
    • بيانات النشر:
      BMC, 2024.
    • الموضوع:
      2024
    • Collection:
      LCC:Biotechnology
      LCC:Genetics
    • نبذة مختصرة :
      Abstract Diabetes cell replacement therapy has the potential to be transformed by human pluripotent stem cell-derived β cells (SC-β cells). However, the precise identity of SC-β cells in relationship to primary fetal and adult β-cells remains unclear. Here, we used single-cell sequencing datasets to characterize the transcriptional identity of islets from in vitro differentiation, fetal islets, and adult islets. Our analysis revealed that SC-β cells share a core β-cell transcriptional identity with human adult and fetal β-cells, however SC-β cells possess a unique transcriptional profile characterized by the persistent expression and activation of progenitor and neural-biased gene networks. These networks are present in SC-β cells, irrespective of the derivation protocol used. Notably, fetal β-cells also exhibit this neural signature at the transcriptional level. Our findings offer insights into the transcriptional identity of SC-β cells and underscore the need for further investigation of the role of neural transcriptional networks in their development.
    • File Description:
      electronic resource
    • ISSN:
      1471-2164
    • Relation:
      https://doaj.org/toc/1471-2164
    • الرقم المعرف:
      10.1186/s12864-024-10013-x
    • الرقم المعرف:
      edsdoj.0e2562a64d84edba75d1a2a6353b1ca