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An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels

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اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • بيانات النشر:
      Fundação Oswaldo Cruz (FIOCRUZ), 1990.
    • الموضوع:
      1990
    • Collection:
      LCC:Microbiology
      LCC:Infectious and parasitic diseases
    • نبذة مختصرة :
      A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels.
    • File Description:
      electronic resource
    • ISSN:
      0074-0276
      1678-8060
    • Relation:
      http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000100017; https://doaj.org/toc/0074-0276; https://doaj.org/toc/1678-8060
    • الرقم المعرف:
      10.1590/S0074-02761990000100017
    • الرقم المعرف:
      edsdoj.0a5ba176215548eb84908b65af188a1e